GIRK2 Recombinant Rabbit Monoclonal Antibody [PSH05-91]
Catalog# HA722478
GIRK2 Recombinant Rabbit Monoclonal Antibody [PSH05-91]
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WB
-
IHC-P
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IP
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IHC-Fr
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Human
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Mouse
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Rat
概述
产品名称
GIRK2 Recombinant Rabbit Monoclonal Antibody [PSH05-91]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IP, IHC-Fr
分子量
Predicted band size: 48 kDa
阳性对照
Mouse brain tissue lysate, mouse hippocampus tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, mouse brain tissue.
偶联
unconjugated
克隆号
PSH05-91
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IHC-P
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1:1,000
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IP
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1-2μg/sample
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IHC-Fr
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1:200
靶点
功能
This potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This gene encodes a member of the G protein-coupled inwardly-rectifying potassium channel family of inward rectifier potassium channels. This type of potassium channel allows a greater flow of potassium into the cell than out of it. These proteins modulate many physiological processes, including heart rate in cardiac cells and circuit activity in neuronal cells, through G-protein coupled receptor stimulation. Mutations in this gene are associated with Keppen-Lubinsky Syndrome, a rare condition characterized by severe developmental delay, facial dysmorphism, and intellectual disability.
背景文献
1. Reyes S., Fu Y., Double K., Thompson L., Kirik D., Paxinos G., Halliday G.M. GIRK2 expression in dopamine neurons of the substantia nigra and ventral tegmental area. J Comp Neurol 520:2591-2607 (2012)
2. Cramer N.P., Best T.K., Stoffel M., Siarey R.J., Galdzicki Z. GABAB-GIRK2-mediated signaling in Down syndrome. Adv Pharmacol 58:397-426 (2010)
亚细胞定位
Membrane.
别名
inwardly rectifying subfamily J member 6 antibody
BIR1 antibody
G protein activated inward rectifier potassium channel 2 antibody
G protein-activated inward rectifier potassium channel 2 antibody
GIRK-2 antibody
GIRK2 antibody
hiGIRK2 antibody
Inward rectifier K(+) channel Kir3.2 antibody
Inward rectifier potassium channel KIR3.2 antibody
KATP-2 antibody
展开图片
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Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-GIRK2 antibody (HA722478) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722478, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Western blot analysis of GIRK2 on different lysates with Rabbit anti-GIRK2 antibody (HA722478) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Mouse hippocampus tissue lysate
Lane 3: Rat brain tissue lysate (no heat)
Lane 4: Rat hippocampus tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 35/48 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722478) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
GIRK2 was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA722478 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722478 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate (input)
Lane 2: HA722478 IP in mouse brain tissue lysate
Lane 3: Rabbit IgG instead of HA722478 in mouse brain tissue lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 26 seconds; ECL: K1801 -
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GIRK2 antibody (HA722478) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722478) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GIRK2 antibody (HA722478) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722478) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-GIRK2 antibody (HA722478) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722478) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GIRK2 antibody (HA722478) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722478) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Rabbit anti-GIRK2 antibody (HA722478) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722478) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"