Delta-catenin Recombinant Rabbit Monoclonal Antibody [JE75-34]
Catalog# HA722438
Delta-catenin Recombinant Rabbit Monoclonal Antibody [JE75-34]
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WB
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IHC-P
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IP
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IHC-Fr
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Human
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Mouse
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Rat
概述
产品名称
Delta-catenin Recombinant Rabbit Monoclonal Antibody [JE75-34]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Mouse Catenin delta-2 aa 1-100 / 1,247.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IP, IHC-Fr
分子量
Predicted band size: 135 kDa
阳性对照
Mouse brain tissue lysate, mouse hippocampus tissue lysate, mouse cerebellum tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, rat cerebellum tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue.
偶联
unconjugated
克隆号
JE75-34
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IHC-P
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1:200
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IP
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1-2μg/sample
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IHC-Fr
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1:200
靶点
功能
δ-Catenin is a subfamily of catenin proteins with ten armadillo-repeats and includes the proteins catenin delta-1 and catenin delta-2. Catenin delta-2 is expressed in the brain where it is important for normal cognitive development. Like β-catenin and γ-catenin, δ-catenins seem to interact with presenilins. These catenin-presenilin interaction have implications for cadherin function and regulation of cell-to-cell adhesion. While β-catenin acts as a transcription regulatory protein in the Wnt/TCF pathway, delta-1 catenin has been implicated as a regulator of the NF-κB transcription factor. Palmitoylation of δ-catenin seems to coordinate activity-dependent changes in synaptic adhesion molecules, synapse structure, and receptor localizations that are involved in memory formation.
背景文献
1. Zang X et al. Circular RNA EIF4G3 suppresses gastric cancer progression through inhibition of beta-catenin by promoting delta-catenin ubiquitin degradation and upregulating SIK1. Mol Cancer. 2022 Jul
2. Tan CX et al. delta-Catenin controls astrocyte morphogenesis via layer-specific astrocyte-neuron cadherin interactions. J Cell Biol. 2023 Nov
亚细胞定位
Nucleus, Cell junction, adherens junction, Cell projection, dendrite, Perikaryon.
别名
ARM-repeat protein antibody
Catenin (cadherin associated protein) delta 2 antibody
catenin (cadherin-associated protein), delta 2 (neural plakophilin-related arm-repeat protein) antibody
Catenin delta 2 antibody
Catenin delta-2 antibody
CTND2_HUMAN antibody
CTNND 2 antibody
Ctnnd2 antibody
Delta catenin antibody
Delta-catenin antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of Delta-catenin on different lysates with Rabbit anti-Delta-catenin antibody (HA722438) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse hippocampus tissue lysate
Lane 3: Mouse heart tissue lysate (negative)
Lane 4: Mouse kidney tissue lysate (negative)
Lane 5: Mouse cerebellum tissue lysate
Lane 6: Rat brain tissue lysate
Lane 7: Rat heart tissue lysate (negative)
Lane 8: Rat kidney tissue lysate (negative)
Lane 9: Rat spleen tissue lysate (negative)
Lane 10: Rat hippocampus tissue lysate
Lane 11: Rat cerebellum tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 135 kDa
Observed band size: 135 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722438) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Delta-catenin was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA722438 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722438 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate (input)
Lane 2: HA722438 IP in mouse brain tissue lysate
Lane 3: Rabbit IgG instead of HA722438 in mouse brain tissue lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 17 seconds; ECL: K1801 -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Delta-catenin antibody (HA722438) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722438) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Delta-catenin antibody (HA722438) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722438) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Delta-catenin antibody (HA722438) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722438) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-Delta-catenin antibody (HA722438) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722438, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"