IQGAP2 Recombinant Rabbit Monoclonal Antibody [JE64-93]
Catalog# HA721096
IQGAP2 Recombinant Rabbit Monoclonal Antibody [JE64-93]
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WB
-
IHC-P
-
Human
概述
产品名称
IQGAP2 Recombinant Rabbit Monoclonal Antibody [JE64-93]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human IQGAP2 aa 1,301-1,500/1,575.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 181 kDa
阳性对照
Human placenta tissue lysates, human liver tissue lysate, Jurkat cell lysate, human gallbladder tissue, human prostate tissue.
偶联
unconjugated
克隆号
JE64-93
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:5,000
-
IHC-P
-
1:100
靶点
功能
Ras GTPase-activating-like protein IQGAP2 is an enzyme that in humans is encoded by the IQGAP2 gene. This gene encodes a member of the IQGAP family. The encoded protein contains three IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. This protein interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. It also acts as a tumor suppressor and has been found to play a role in regulating innate antiviral responses. Alternative splicing results in multiple transcript variants. IQGAP2 has been shown to interact with CDC42 and RAC1.
背景文献
1. de Ligt J., et al. Diagnostic exome sequencing in persons with severe intellectual disability. N. Engl. J. Med. 367:1921-1929(2012).
2. Kumar D, et al. Reduced expression of IQGAP2 and higher expression of IQGAP3 correlates with poor prognosis in cancers. PLoS One. 2017 Oct 26;12(10):e0186977.
亚细胞定位
Actin cytoskeleton, microtubule, cytosol, extracellular exosome, plasma membrane, cell surface, cytoplasm, filopodium, lamellipodium, microvillus, secretory granule membrane
UNIPROT #
别名
IQ motif containing GTPase activating protein 2 antibody
IQGA2_HUMAN antibody
Iqgap2 antibody
Ras GTPase activating-like protein antibody
Ras GTPase-activating-like protein IQGAP2 antibody
图片
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Western blot analysis of IQGAP2 on human placenta tissue lysates with Rabbit anti-IQGAP2 antibody (HA721096) at 1/5,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 181 kDa
Observed band size: 181 kDa
Exposure time: 1 minute;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721096) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of IQGAP2 on different lysates with Rabbit anti-IQGAP2 antibody (HA721096) at 1/1,000 dilution.
Lane 1: Human liver tissue lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (10 µg/Lane)
Predicted band size: 181 kDa
Observed band size: 181 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721096) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-IQGAP2 antibody (HA721096) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721096) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-IQGAP2 antibody (HA721096) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721096) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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