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USO1 Recombinant Rabbit Monoclonal Antibody [JE64-48]

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Catalog# HA721060

USO1 Recombinant Rabbit Monoclonal Antibody [JE64-48]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

概述

产品名称

USO1 Recombinant Rabbit Monoclonal Antibody [JE64-48]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within human USO1 aa 1-50/962.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Cell, IHC-P

分子量

Predicted band size: 108 kDa

阳性对照

Hela cell lysate, 293T cell lysate, HepG2 cell lysate, A431 cell lysate, NIH/3T3 cell lysates, PC-12 cell lysates, rat epididymis tissue, human testis tissue, mouse cerebellum tissue, SH-SY5Y.

偶联

unconjugated

克隆号

JE64-48

RRID

AB_3072184

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

  • IF-Cell

  • 1:400

  • IHC-P

  • 1:100

靶点

功能

General vesicular transport factor p115 is a protein that in humans is encoded by the USO1 gene. The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum (ER), where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GOLGA2, and giantin in a sequential manner.

背景文献

1. Yoon S. et. al. USO1 isoforms differentially promote liver cancer progression by dysregulating the ER-Golgi network. Carcinogenesis. 2021 Oct

2. Heo Y. et. al. Crystal structures of Uso1 membrane tether reveal an alternative conformation in the globular head domain. Sci Rep. 2020 Jun

亚细胞定位

Cytosol, Golgi apparatus membrane.

UNIPROT #

SwissProt: O60763 Human

SwissProt: Q9Z1Z0 Mouse

SwissProt: P41542 Rat

别名

General vesicular transport factor antibody

General vesicular transport factor p115 antibody

P115 antibody

Protein USO1 homolog antibody

TAP antibody

Transcytosis associated protein antibody

Transcytosis-associated protein antibody

Uso1 antibody

USO1_HUMAN antibody

VDP antibody

展开

图片

  • Western blot analysis of USO1 on different lysates with Rabbit anti-USO1 antibody (<a href="/products/HA721060" style="font-weight: bold;text-decoration: underline;">HA721060</a>) at 1/1,000 dilution.<br /><br />Lane 1: Hela cell lysate<br />Lane 2: 293T cell lysate<br />Lane 3: HepG2 cell lysate<br />Lane 4: A431 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 108 kDa<br />Observed band size: 108 kDa<br /><br />Exposure time: 1 minute;<br /><br />6% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721060" style="font-weight: bold;text-decoration: underline;">HA721060</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of USO1 on different lysates with Rabbit anti-USO1 antibody (HA721060) at 1/1,000 dilution.

    Lane 1: Hela cell lysate
    Lane 2: 293T cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: A431 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 108 kDa
    Observed band size: 108 kDa

    Exposure time: 1 minute;

    6% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721060) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of USO1 on NIH/3T3 cell lysates with Rabbit anti-USO1 antibody (<a href="/products/HA721060" style="font-weight: bold;text-decoration: underline;">HA721060</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 108 kDa<br />Observed band size: 108 kDa<br /><br />Exposure time: 1 minute;<br /><br />6% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721060" style="font-weight: bold;text-decoration: underline;">HA721060</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of USO1 on NIH/3T3 cell lysates with Rabbit anti-USO1 antibody (HA721060) at 1/1,000 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 108 kDa
    Observed band size: 108 kDa

    Exposure time: 1 minute;

    6% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721060) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of USO1 on PC-12 cell lysates with Rabbit anti-USO1 antibody (<a href="/products/HA721060" style="font-weight: bold;text-decoration: underline;">HA721060</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 108 kDa<br />Observed band size: 108 kDa<br /><br />Exposure time: 1 minute;<br /><br />6% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721060" style="font-weight: bold;text-decoration: underline;">HA721060</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of USO1 on PC-12 cell lysates with Rabbit anti-USO1 antibody (HA721060) at 1/1,000 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 108 kDa
    Observed band size: 108 kDa

    Exposure time: 1 minute;

    6% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721060) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-USO1 antibody (<a href="/products/HA721060" style="font-weight: bold;text-decoration: underline;">HA721060</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721060" style="font-weight: bold;text-decoration: underline;">HA721060</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-USO1 antibody (HA721060) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721060) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-USO1 antibody (<a href="/products/HA721060" style="font-weight: bold;text-decoration: underline;">HA721060</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721060" style="font-weight: bold;text-decoration: underline;">HA721060</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-USO1 antibody (HA721060) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721060) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-USO1 antibody (<a href="/products/HA721060" style="font-weight: bold;text-decoration: underline;">HA721060</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721060" style="font-weight: bold;text-decoration: underline;">HA721060</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-USO1 antibody (HA721060) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721060) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry analysis of SH-SY5Y cells labeling USO1 with Rabbit anti-USO1 antibody (<a href="/products/HA721060" style="font-weight: bold;text-decoration: underline;">HA721060</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-USO1 antibody (<a href="/products/HA721060" style="font-weight: bold;text-decoration: underline;">HA721060</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 647, <a href="/products/HA1127" style="font-weight: bold;text-decoration: underline;">HA1127</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of SH-SY5Y cells labeling USO1 with Rabbit anti-USO1 antibody (HA721060) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-USO1 antibody (HA721060) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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