GFAP Mouse Monoclonal Antibody [PSH05-17] - BSA and Azide free (Detector)
概述
产品名称
GFAP Mouse Monoclonal Antibody [PSH05-17] - BSA and Azide free (Detector)
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within C-terminal human GFAP.
种属反应性
Human, Mouse, Rat
验证应用
ELISA(Det)
分子量
Predicted band size: 50 kDa
阳性对照
Recombinant human GFAP protein (HA210990).
偶联
unconjugated
克隆号
PSH05-17
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [PSH05-16] to GFAP (Capture) (HA722234) and recombinant Human GFAP protein (HA210990) as the standard. The reference range value is 823-200,000pg/ml.
靶点
功能
Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans. It is a type III intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS), including astrocytes and ependymal cells during development. GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts taken from rat kidneys,[8] Leydig cells of the testis in both hamsters and humans, human keratinocytes, human osteocytes and chondrocytes and stellate cells of the pancreas and liver in rats. GFAP is closely related to the other three non-epithelial type III IF family members, vimentin, desmin and peripherin, which are all involved in the structure and function of the cell's cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength as well as the shape of cells, but its exact function remains poorly understood, despite the number of studies using it as a cell marker.
背景文献
1. Abdelhak A et al. Blood GFAP as an emerging biomarker in brain and spinal cord disorders. Nat Rev Neurol. 2022 Mar
2. Kim KY et al. GFAP as a Potential Biomarker for Alzheimer\'s Disease: A Systematic Review and Meta-Analysis. Cells. 2023 May
亚细胞定位
Cytoplasm.
别名
wu:fb34h11 antibody
ALXDRD antibody
cb345 antibody
etID36982.3 antibody
FLJ42474 antibody
FLJ45472 antibody
GFAP antibody
GFAP_HUMAN antibody
gfapl antibody
Glial fibrillary acidic protein antibody
展开图片
-
Sandwich ELISA analysis of Human GFAP matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722234) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant human GFAP protein (HA210990) starting from 100000 pg/ml to 0 pg/ml and detect antibody (HA601342, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
The concentrations of GFAP were interpolated from the GFAP standard curve and corrected for sample dilution. The mean GFAP concentration was determined to be 41.29 ng/ml in rat brain tissue extract. There was no detectable signal in 293T and NIH/3T3 cell extract.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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