RPS6 Recombinant Mouse Monoclonal Antibody [A6B8-R]
Catalog# HA601324
RPS6 Recombinant Mouse Monoclonal Antibody [A6B8-R]
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WB
-
Human
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Mouse
-
Rat
概述
产品名称
RPS6 Recombinant Mouse Monoclonal Antibody [A6B8-R]
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within human RPS6 aa 1-150.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 29 kDa
阳性对照
HeLa cell lysate, MCF7 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, HCT 116 cell lysate, THP-1 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate.
偶联
unconjugated
克隆号
A6B8-R
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000
靶点
功能
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
背景文献
1. Yan M. et. al. mTORC1/rpS6 signaling complex modifies BTB transport function: an in vivo study using the adjudin model. Am J Physiol Endocrinol Metab. 2019 Jul
2. Wu S. et. al. mTORC1/rpS6 and spermatogenic function in the testis-insights from the adjudin model. Reprod Toxicol. 2019 Oct
亚细胞定位
Cytosol, Endoplasmic reticulum, Nucleus.
别名
40S ribosomal protein S6 antibody
Air8 antibody
NP33 antibody
Phosphoprotein NP33 antibody
Pp30 antibody
Ribosomal protein S6 antibody
RP S6 antibody
rps6 antibody
RS6 antibody
RS6_HUMAN antibody
展开图片
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Western blot analysis of RPS6 on different lysates with Mouse anti-RPS6 antibody (HA601324) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: HCT 116 cell lysate
Lane 6: THP-1 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 1 minute 22 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601324) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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