WLS Mouse Monoclonal Antibody [A7C2]
Catalog# HA600060
WLS Mouse Monoclonal Antibody [A7C2]
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WB
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IF-Cell
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Human
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Mouse
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Rat
概述
产品名称
WLS Mouse Monoclonal Antibody [A7C2]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within human WLS aa 50-200/541.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell
分子量
Predicted band size: 62 kDa
阳性对照
SH-SY5Y cell lysate, Hela cell lysate, mouse lung tissue lysate, rat brain tissue lysate, Hela, SH-SY5Y.
偶联
unconjugated
克隆号
A7C2
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG2b
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:5,000-1:20,000
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IF-Cell
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1:100
靶点
功能
Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity).
背景文献
1. Zheng D. et. al. Wntless (Wls): A Prognostic Index for Progression and Patient Survival of Breast Cancer. Onco Targets Ther. 2020 Dec
2. Cheng J. et. al. Conditional deletion of Wntless in granulosa cells causes impaired corpora lutea formation and subfertility. Aging (Albany NY). 2020 Dec
亚细胞定位
Golgi apparatus membrane, Golgi apparatus membrane, Early endosome membrane, Endoplasmic reticulum membrane, Cell membrane, Cytoplasmic vesicle membrane.
别名
C1orf139 antibody
DKFZp686I0788 antibody
Evenness interrupted, Drosophila, homolg of antibody
EVI antibody
FLJ23091 antibody
G protein coupled receptor 177 antibody
GPR 177 antibody
Integral membrane protein GPR177 antibody
MGC131760 antibody
MGC14878 antibody
展开图片
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Western blot analysis of WLS on different lysates with Mouse anti-WLS antibody (HA600060) at 1/20,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: Hela cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 55 kDa
Exposure time: 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600060) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of WLS on different lysates with Mouse anti-WLS antibody (HA600060) at 1/5,000 dilution.
Lane 1: Mouse lung tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 55 kDa
Exposure time: 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600060) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Hela cells labeling WLS with Mouse anti-WLS antibody (HA600060) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-WLS antibody (HA600060) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunocytochemistry analysis of SH-SY5Y cells labeling WLS with Mouse anti-WLS antibody (HA600060) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-WLS antibody (HA600060) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"