RNF111 Rabbit Polyclonal Antibody
Catalog# HA500485
RNF111 Rabbit Polyclonal Antibody
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WB
-
IHC-P
-
FC
-
Human
概述
产品名称
RNF111 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within human RNF111 aa 795-994 / 994.
种属反应性
Human
验证应用
WB, IHC-P, FC
分子量
109 kDa
阳性对照
K562 cell lysate, 293T cell lysate, Hela cell lysate, human placenta tissue, Hela.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:500-1:2,000
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IHC-P
-
1:50-1:200
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FC
-
1:500-1:1,000
靶点
功能
E3 ubiquitin-protein ligase. Required for mesoderm patterning during embryonic development. Acts as an enhancer of the transcriptional responses of the SMAD2/SMAD3 effectors, which are activated downstream of BMP. Acts by mediating ubiquitination and degradation of SMAD inhibitors such as SMAD7, inducing their proteasomal degradation and thereby enhancing the transcriptional activity of TGF-beta and BMP. In addition to enhance transcription of SMAD2/SMAD3 effectors, also regulates their turnover by mediating their ubiquitination and subsequent degradation, coupling their activation with degradation, thereby ensuring that only effectors 'in use' are degraded. Activates SMAD3/SMAD4-dependent transcription by triggering signal-induced degradation of SNON isoform of SKIL. Associates with UBE2D2 as an E2 enzyme. Specifically binds polysumoylated chains via SUMO interaction motifs (SIMs) and mediates ubiquitination of sumoylated substrates. Catalyzes 'Lys-63'-linked ubiquitination of sumoylated XPC in response to UV irradiation, promoting nucleotide excision repair. Mediates ubiquitination and degradation of sumoylated PML. The regulation of the BMP-SMAD signaling is however independent of sumoylation and is not dependent of SUMO interaction motifs (SIMs).
背景文献
1. Wang Z. et. al. circRNA RNF111 regulates the growth, migration and invasion of gastric cancer cells by binding to miR-27b-3p. Int J Mol Med. 2020 Nov
2. Zang H. et. al. Circ-RNF111 contributes to paclitaxel resistance in breast cancer by elevating E2F3 expression via miR-140-5p. Thorac Cancer. 2020 Jul
亚细胞定位
Cytoplasm, Nucleus, PML body.
UNIPROT #
别名
BCA2 antibody
Breast cancer associated gene 2 antibody
CL469780 antibody
E3 ubiquitin-protein ligase RNF115 antibody
Rabring 7 antibody
RING finger protein 115 antibody
RN115_HUMAN antibody
RNF115 antibody
Zinc finger protein 364 antibody
ZNF364 antibody
图片
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Western blot analysis of RNF111 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500485, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: 293T cell lysate
Lane 3: Hela cell lysate -
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-RNF111 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500485, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of Hela cells labeling RNF111.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500485, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"