UGGT1 Rabbit Polyclonal Antibody
Catalog# HA500025
UGGT1 Rabbit Polyclonal Antibody
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WB
-
IHC-P
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Human
-
Mouse
-
Rat
概述
产品名称
UGGT1 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within human UGGT1 aa 1350-1555.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
177 kDa
阳性对照
Rat liver tissue lysate, HL-60 cell lysate, mouse liver tissue lysate, mouse brain tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
靶点
功能
UGGT is part of the ER quality control system of glycoprotein folding and its activity increases the potential for correctly folded glycoproteins.The main proteins involved in the ER quality control system are UGGT, the ER lectin chaperones (calnexin and calreticulin), and glucosidase II. UGGT first recognizes the incompletely folded glycoprotein and monoglucosylates it. The lectins, calnexin and calreticulin, have high affinities for monoglucosylated proteins and the ER chaperones that associate with these lectins assist the folding of the misfolded glycoprotein. Subsequently, glucosidase II will deglucosylate the glycoprotein. If the glycoprotein is still misfolded, UGGT will re-glucosylate it and allow it to go through the cycle again.
背景文献
1. Arnold S.M. et. al. Two homologues encoding human UDP-glucose:glycoprotein glucosyltransferase differ in mRNA expression and enzymatic activity. Biochemistry 39:2149-2163(2000).
亚细胞定位
Endoplasmic reticulum.
别名
GT antibody
HUGT1 antibody
UDP glucose glycoprotein glucosyltransferase 1 antibody
UDP Glucose Glycoprotein Glucosyltransferase antibody
UDP--Glc:glycoprotein glucosyltransferase antibody
UDP-glucose ceramide glucosyltransferase-like 1 antibody
UDP-glucose:glycoprotein glucosyltransferase 1 antibody
UGCGL1 antibody
UGGG1 antibody
UGGG1_HUMAN antibody
展开图片
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Western blot analysis of UGGT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500025, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat liver tissue lysate
Lane 2: HL-60 cell lysate
Lane 3: Mouse liver tissue lysate -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-UGGT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500025, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"