概述
产品名称
Lamin A + Lamin C Recombinant Rabbit Monoclonal Antibody [JE51-60]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Lamin A / C aa 191-309 / 664.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IHC-P, FC
分子量
Predicted band size: 74 kDa
阳性对照
HeLa cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse ovary tissue lysate, Rat ovary tissue lysate, HeLa, human skin tissue, human breast tissue, human colon carcinoma tissue, human colon tissue, human tonsil tissue, human gastric carcinoma tissue, mouse brain tissue, mouse colon tissue, rat colon tissue.
偶联
unconjugated
克隆号
JE51-60
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IF-Cell
-
1:200
-
IHC-P
-
1:50-1:2,000
-
FC
-
1:1,000
发表文章中的应用
发表文章中的种属
Human | See 3 publications below |
Mouse | See 1 publications below |
靶点
功能
A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/ICE, is comprised of ICE, CPP32, ICH-1/Nedd-2, Tx, Mch2, Mch3 (ICE-LAP3 or CMH-1), Mch4 and ICE-LAP6. Ced-3/ICE family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology. The nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family. Lamin C is a splice variant of Lamin A, differing only at the carboxy-terminus. Lamins A and C are identical for the first 566 amino acids, with Lamin C differing only in six unique carboxy-terminal amino acids.
背景文献
1. Jimenez-Escrig A. et. al. Autosomal recessive Emery-Dreifuss muscular dystrophy caused by a novel mutation (R225Q) in the lamin A/C gene identified by exome sequencing. Muscle Nerve 45:605-610(2012).
2. Renou L. et. al. Heart-hand syndrome of Slovenian type: a new kind of laminopathy. J. Med. Genet. 45:666-671(2008).
序列相似性
Belongs to the intermediate filament family.
组织特异性
In the arteries, prelamin-A/C accumulation is not observed in young healthy vessels but is prevalent in medial vascular smooth muscle cells (VSMCs) from aged individuals and in atherosclerotic lesions, where it often colocalizes with senescent and degenerate VSMCs. Prelamin-A/C expression increases with age and disease. In normal aging, the accumulation of prelamin-A/C is caused in part by the down-regulation of ZMPSTE24/FACE1 in response to oxidative stress.
翻译后修饰
Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.; Proteolytic cleavage of the C-terminal of 18 residues of prelamin-A/C results in the production of lamin-A/C. The prelamin-A/C maturation pathway includes farnesylation of CAAX motif, ZMPSTE24/FACE1 mediated cleavage of the last three amino acids, methylation of the C-terminal cysteine and endoproteolytic removal of the last 15 C-terminal amino acids. Proteolytic cleavage requires prior farnesylation and methylation, and absence of these blocks cleavage.; Sumoylation is necessary for the localization to the nuclear envelope.; Farnesylation of prelamin-A/C facilitates nuclear envelope targeting.
亚细胞定位
Intermediate filament, Nucleus.
别名
70 kDa lamin antibody
Cardiomyopathy dilated 1A (autosomal dominant) antibody
CDCD1 antibody
CDDC antibody
CMD1A antibody
CMT2B1 antibody
EMD2 antibody
FPL antibody
FPLD antibody
FPLD2 antibody
展开70 kDa lamin antibody
Cardiomyopathy dilated 1A (autosomal dominant) antibody
CDCD1 antibody
CDDC antibody
CMD1A antibody
CMT2B1 antibody
EMD2 antibody
FPL antibody
FPLD antibody
FPLD2 antibody
HGPS antibody
IDC antibody
Lamin A antibody
Lamin A/C antibody
Lamin A/C like 1 antibody
Lamin antibody
Lamin C antibody
Lamin-A/C antibody
LDP1 antibody
LFP antibody
LGMD1B antibody
Limb girdle muscular dystrophy 1B (autosomal dominant) antibody
LMN 1 antibody
LMN A antibody
LMN C antibody
LMN1 antibody
LMNA antibody
LMNA_HUMAN antibody
LMNC antibody
LMNL1 antibody
Prelamin A/C antibody
PRO1 antibody
Renal carcinoma antigen NY REN 32 antibody
Renal carcinoma antigen NY-REN-32 antibody
Renal carcinoma antigen NYREN32 antibody
折叠图片
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Western blot analysis of Lamin A + Lamin C on different lysates with Rabbit anti-Lamin A + Lamin C antibody (ET7110-12) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: PC-12 cell lysate (20 µg/Lane)
Lane 4: Mouse ovary tissue lysate (40 µg/Lane)
Lane 5: Rat ovary tissue lysate (40 µg/Lane)
Predicted band size: 74 kDa
Observed band size: 70/65 kDa
Exposure time: Lane 1-2: 25 seconds; Lane 3-5: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-12) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling Lamin A + Lamin C with Rabbit anti-Lamin A + Lamin C antibody (ET7110-12) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lamin A + Lamin C antibody (ET7110-12) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-12) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-12) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lamin A + Lamin C antibody (ET7110-12) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-12) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Lamin A + Lamin C antibody (ET7110-12) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-12) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Lamin A + Lamin C antibody (ET7110-12) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-12) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Lamin A + Lamin C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-12, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Lamin A + Lamin C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-12, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Lamin A + Lamin C antibody (ET7110-12) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-12) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Lamin A + Lamin C antibody (ET7110-12) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-12) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of HeLa cells labeling Lamin A + Lamin C.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET7110-12, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Protein arginine methyltransferase 5 mediates arginine symmetric dimethylation of influenza A virus PB2 and supports viral replication
Author:
PMID: 37830751
应用: WB
反应种属:
发表时间: 2023 Oct
-
Citation
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Knockdown of LMNA inhibits Akt/β-catenin-mediated cell invasion and migration in clear cell renal cell carcinoma cells
Author: Xin H, Tang Y, Jin YH, et al
PMID: 37749865
应用: WB
反应种属: Human
发表时间: 2023 Dec
-
Citation
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UM-164, a Dual Inhibitor of c-Src and p38 MAPK, Suppresses Proliferation of Glioma by Reducing YAP Activity
Author: Xu, H., Zhang, Y., Liu, J., Cui, J., Gan, Y., Wu, Z., Chang, Y., Sui, R., Chen, Y., Shi, J., Liang, H., Liu, Q., Sun, S., & Piao, H.
PMID: 36358761
应用: WB
反应种属: Human
发表时间: 2022 Oct
-
Citation
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Aberrant nuclear lamina contributes to the malignancy of human gliomas
Author:
PMID: 34530169
应用: WB
反应种属: Mouse
发表时间: 2022 Feb
-
Citation
-
Transportin-3 Facilitates Uncoating of Influenza A Virus
Author: Zou, J., Yu, L., Zhu, Y., Yang, S., Zhao, J., Zhao, Y., Jiang, M., Xie, S., Liu, H., Zhao, C., & Zhou, H.
PMID: 35456945
应用: WB
反应种属: Human
发表时间: 2022 Apr
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Citation