GST3 Recombinant Rabbit Monoclonal Antibody [JB40-79]
Catalog# ET7107-71
GST3 Recombinant Rabbit Monoclonal Antibody [JB40-79]
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WB
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IHC-P
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IF-Cell
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IF-Tissue
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Human
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Mouse
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Rat
概述
产品名称
GST3 Recombinant Rabbit Monoclonal Antibody [JB40-79]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human GST3aa 70-210 / 210.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Cell, IF-Tissue
分子量
Predicted band size: 23 kDa
阳性对照
A549 cell lysate, mouse liver tissue lysate, A431, A549, LOVO, rat epididymis tissue, human liver tissue, human placenta tissue, mouse fallopian tube tissue,human lung tissue,human lung squamous carcinoma tissue .
偶联
unconjugated
克隆号
JB40-79
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:500
靶点
功能
Glutathione S-transferases (GSTs) function in the metabolic detoxification of various environmental carcinogens and lipid hydroperoxides. In response to oxidative stress, upregulation of the GST family member GSTP1 occurs, consistent with this function. Furthermore, the GSTP1 gene is subject to CpG island hypermethylation, a state that correlates with human prostatic carcinogenesis. GSTP1 gene hypermethylation can be detected in urine, ejaculate and plasma from men with prostate cancer, potentially making GSTP1 a useful biomarker for prostate cancer screening.
背景文献
1. Sun K H et al. Glutathione-S-transferase P1 is a critical regulator of Cdk5 kinase activity. J Neurochem 118:902-914 (2011).
2. Kong K H et al. Tyrosine-7 in human class Pi glutathione S-transferase is important for lowering the pKa of the thiol group of glutathione in the enzyme-glutathione complex. Biochem Biophys Res Commun 184:194-197 (1992).
序列相似性
Belongs to the GST superfamily. Pi family.
亚细胞定位
Cytoplasm, Mitochondrion, Nucleus.
别名
Deafness antibody
Deafness X-linked 7 antibody
DFN7 antibody
FAEES3 antibody
Fatty Acid Ethyl Ester Synthase III antibody
Glutathione S Transferase 3 antibody
Glutathione S Transferase Pi antibody
Glutathione S-transferase P antibody
Glutathione S-transferase pi 1 antibody
GST class-pi antibody
展开图片
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Western blot analysis of GST3 on different lysates with Rabbit anti-GST3 antibody (ET7107-71) at 1/1,000 dilution.
Lane 1: HCT 116 cell lysate
Lane 1: HT-29 cell lysate
Lane 1: K-562 cell lysate
Lane 1: A549 cell lysate
Lane 2: Mouse liver tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane1-4 and 40ug/Lane5-7.
Predicted band size: 23 kDa
Observed band size: 25 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-71) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of GST3 on different lysates with Rabbit anti-GST3 antibody (ET7107-71) at 1/1,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si GST3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-71) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining of GST3 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-71, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of GST3 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-71, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of GST3 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-71, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-GST3 antibody (ET7107-71) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-71) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma tissue with Rabbit anti-GST3 antibody (ET7107-71) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-71) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"