GABRA5 Recombinant Rabbit Monoclonal Antibody [JB34-19]
Catalog# ET7107-08
GABRA5 Recombinant Rabbit Monoclonal Antibody [JB34-19]
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WB
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
GABRA5 Recombinant Rabbit Monoclonal Antibody [JB34-19]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human GABRA5 aa 1-240 / 462.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Tissue, IHC-P
分子量
Predicted band size: 52 kDa
阳性对照
A549 cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse brain tissue lysate, mouse brain tissue, rat brain tissue, mouse cerebellum tissue.
偶联
unconjugated
克隆号
JB34-19
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:2,000
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IF-Tissue
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1:500
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IHC-P
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1:1,000
靶点
功能
Gamma-aminobutyric acid (GABA) A receptor, alpha 5, also known as GABRA5, is a protein which in humans is encoded by the GABRA5 gene. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABAA receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABAA receptor. At least 16 distinct subunits of GABAA receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described. Recent research has produced several ligands which are moderately selective for GABAA receptors containing the α5 subunit. These have proved to be useful in investigating some of the side effects of benzodiazepine and nonbenzodiazepine drugs, particularly the effects on learning and memory such as anterograde amnesia. Inverse agonists at this subunit have nootropic effects and may be useful for the treatment of cognitive disorders such as Alzheimer's disease.
背景文献
1. Denis N J et al. Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry. Proteomics 7:868-874 (2007).
2. Kaneko Y et al. Oxytocin modulates GABAAR subunits to confer neuroprotection in stroke in vitro. Sci Rep 6:35659 (2016).
序列相似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA5 sub-subfamily.
亚细胞定位
Postsynaptic cell membrane, Cell membrane.
别名
GAA 5 antibody
GAA5 antibody
GABA(A) receptor subunit alpha-5 antibody
GABRA 5 antibody
Gabra5 antibody
Gamma aminobutyric acid GABA A receptor alpha 5 antibody
Gamma aminobutyric acid GABA A receptor alpha 5 precursor antibody
Gamma aminobutyric acid receptor alpha 5 subunit precursor GABA A receptor antibody
Gamma-aminobutyric acid receptor subunit alpha-5 antibody
GBRA5_HUMAN antibody
展开图片
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Western blot analysis of GABRA5 on different lysates with Rabbit anti-GABRA5 antibody (ET7107-08) at 1/2,000 dilution.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: Neuro-2a cell lysate (20 µg/Lane)
Lane 3: C6 cell lysate (20 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 52 kDa
Observed band size: 70 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-08) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABRA5 antibody (ET7107-08) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-08) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GABRA5 antibody (ET7107-08) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-08) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABRA5 with Rabbit anti-GABRA5 antibody (ET7107-08) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7107-08, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"