ALDH1L1 Recombinant Rabbit Monoclonal Antibody [JU53-54]
Catalog# ET7106-64
ALDH1L1 Recombinant Rabbit Monoclonal Antibody [JU53-54]
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WB
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IHC-P
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IF-Tissue
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IHC-Fr
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Human
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Mouse
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Rat
概述
产品名称
ALDH1L1 Recombinant Rabbit Monoclonal Antibody [JU53-54]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human ALDH1L1 aa 155-375 .
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Tissue, IHC-Fr
分子量
Predicted band size: 99 kDa
阳性对照
A431, A549, LOVO, human liver tissue, human kidney tissue, mouse liver tissue, rat kidney tissue, rat brain tissue, mouse brain tissue.
偶联
unconjugated
克隆号
JU53-54
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-2,000
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IHC-P
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1:200-1:1,000
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IF-Tissue
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1:50-1:200
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IHC-Fr
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1:500
靶点
功能
10-formyltetrahydrofolate dehydrogenase is an enzyme that in humans is encoded by the ALDH1L1 gene. The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.
背景文献
1. Tsybovsky Y et al. Structure of putative tumor suppressor ALDH1L1. Commun Biol. 2022 Jan
2. Krupenko NI et al. Knockout of Putative Tumor Suppressor Aldh1l1 in Mice Reprograms Metabolism to Accelerate Growth of Tumors in a Diethylnitrosamine (DEN) Model of Liver Carcinogenesis. Cancers (Basel). 2021 Jun
序列相似性
In the N-terminal section; belongs to the GART family.; In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
组织特异性
Highly expressed in liver, pancreas and kidney.
亚细胞定位
Cytoplasm.
别名
10 formyltetrahydrofolate dehydrogenase antibody
10 FTHFDH antibody
10-formyltetrahydrofolate dehydrogenase antibody
10-FTHFDH antibody
AL1L1_HUMAN antibody
Aldehyde dehydrogenase 1 family member L1 antibody
Aldehyde dehydrogenase family 1 member L1 antibody
aldh1l1 antibody
Cytosolic 10-formyltetrahydrofolate dehydrogenase antibody
DKFZp781N0997 antibody
展开图片
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Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7106-64, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7106-64, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling ALDH1L1 with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7106-64, green) at 1/50 dilution overnight at 4 ℃, washed with PBS.
Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling ALDH1L1 with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7106-64, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.
Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ALDH1L1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ALDH1L1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ALDH1L1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ALDH1L1 antibody. Counter stained with hematoxylin.
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Western blot analysis of ALDH1L1 on mouse liver tissue lysates using anti-ALDH1L1 antibody at 1/500 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"