NeuroD1 Recombinant Rabbit Monoclonal Antibody [JM11-10]
Catalog# ET1703-73
NeuroD1 Recombinant Rabbit Monoclonal Antibody [JM11-10]
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WB
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IP
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IHC-P
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IHC-Fr
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Human
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Mouse
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Rat
概述
产品名称
NeuroD1 Recombinant Rabbit Monoclonal Antibody [JM11-10]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human NeuroD1 aa 1-44 / 356.
种属反应性
Human, Mouse, Rat
验证应用
WB, IP, IHC-P, IHC-Fr
分子量
Predicted band size: 40 kDa
阳性对照
Rat brain tissue, mouse cerebellum tissue, rat cerebellum tissue, Human brain tissue lysate, SH-SY5Y cell lysate.
偶联
unconjugated
克隆号
JM11-10
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IP
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1:10-1:50
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IHC-P
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1:200-1:500
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IHC-Fr
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1:100
靶点
功能
The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. The HLH domain is required for dimerization, while the basic region makes specific contacts with DNA. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains. These proteins heterodimerize with members of the E protein family and initiate myogenesis. Neuro D has been identified as a bHLH transcription factor functioning in neurogenic differentiation. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.
背景文献
1. Zhang F et al. Phosphofructokinase-1 Negatively Regulates Neurogenesis from Neural Stem Cells. Neurosci Bull 32:205-16 (2016).
2. Borromeo MD et al. ASCL1 and NEUROD1 Reveal Heterogeneity in Pulmonary Neuroendocrine Tumors and Regulate Distinct Genetic Programs. Cell Rep 16:1259-72 (2016).
翻译后修饰
Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth. Phosphorylated by MAPK1; phosphorylation regulates heterodimerization and DNA-binding activities. Phosphorylation on Ser-266 and Ser-274 increases transactivation on the insulin promoter in glucose-stimulated insulinoma cells (By similarity).
亚细胞定位
Cytoplasm. Nucleus.
别名
atonal antibody
basic helix loop helix transcription factor antibody
BETA 2 antibody
Beta cell E box transactivator 2 antibody
BETA2 antibody
BHF 1 antibody
BHF1 antibody
bHLHa3 antibody
class A basic helix loop helix protein 3 antibody
Class A basic helix-loop-helix protein 3 antibody
展开图片
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Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-NeuroD1 antibody (ET1703-73) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-73, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of frozen rat cerebellum tissue with Rabbit anti-NeuroD1 antibody (ET1703-73) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-73, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-NeuroD1 antibody (ET1703-73) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-73) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-NeuroD1 antibody (ET1703-73) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-73) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-NeuroD1 antibody (ET1703-73) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-73) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of NeuroD1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human brain tissue lysate
Lane 2: SH-SY5Y cell lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"