Parvalbumin Recombinant Rabbit Monoclonal Antibody [JM100-08]
Catalog# ET1703-15
Parvalbumin Recombinant Rabbit Monoclonal Antibody [JM100-08]
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
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IP
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Human
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Mouse
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Rat
概述
产品名称
Parvalbumin Recombinant Rabbit Monoclonal Antibody [JM100-08]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Paravalbumin aa 1-110 / 110.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IHC-Fr, IF-Tissue, IP
分子量
Predicted band size: 12 kDa
阳性对照
RPMI 8226 cell lysate, human kidney tissue, mouse kidney tissue, mouse cerebellum tissue, rat cerebellum tissue.
偶联
unconjugated
克隆号
JM100-08
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IHC-P
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1:200-1:1,000
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IHC-Fr
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1:500
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IF-Tissue
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1:200
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IP
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1-2μg/sample
靶点
功能
Parvalbumin (PV) is a calcium-binding protein with low molecular weight (typically 9-11 kDa). In humans, it is encoded by the PVALB gene. It is not a member of the albumin family; it is named for its size (parv-, from Latin parvus small) and its ability to coagulate. It has three EF hand motifs and is structurally related to calmodulin and troponin C. Parvalbumin is found in fast-contracting muscles, where its levels are highest, as well as in the brain and some endocrine tissues. Parvalbumin is a small, stable protein containing EF-hand type calcium binding sites. It is involved in calcium signaling. Calcium binding proteins like parvalbumin play a role in many physiological processes, namely cell-cycle regulation, second messenger production, muscle contraction, organization of microtubules and phototransduction. Therefore, calcium-binding proteins must distinguish calcium in the presence of high concentrations of other metal ions. The mechanism for the calcium selectivity has been extensively studied.
背景文献
1. Cornez G et al. Anatomically discrete sex differences in neuroplasticity in zebra finches as reflected by perineuronal nets. PLoS One 10:e0123199 (2015).
2. Whissell PD et al. Comparative density of CCK- and PV-GABA cells within the cortex and hippocampus. Front Neuroanat 9:124 (2015).
序列相似性
Belongs to the parvalbumin family.
亚细胞定位
Axon, cytoplasm, synapse.
别名
D22S749 antibody
MGC116759 antibody
Parvalbumin alpha antibody
PRVA_HUMAN antibody
PVALB antibody
图片
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Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/500 dilution.
The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-15, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
SLC32A1 (HA601384, green) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. -
Immunofluorescence analysis of frozen rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/500 dilution.
The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-15, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
SLC32A1 (HA601384, green) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. -
Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/500 dilution.
Important Notice: Antigen retrieval is not required before IHC-Fr staining.
The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-15, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of frozen rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/500 dilution.
Important Notice: Antigen retrieval is not required before IHC-Fr staining.
The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-15, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of Parvalbumin on different lysates with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/1,000 dilution.
Lane 1: RPMI 8226 cell lysate
Lane 2: SK-MEL-28 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 12 kDa
Observed band size: 14 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-15) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling Parvalbumin with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-15, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Parvalbumin was immunoprecipitated from 0.2 mg RPMI 8226 cell lysate with ET1703-15 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET1703-15 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: RPMI 8226 cell lysate (input)
Lane 2: ET1703-15 IP in RPMI 8226 cell lysate
Lane 3: Rabbit IgG instead of ET1703-15 in RPMI 8226 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 10 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"