JNK1+JNK2+JNK3 Recombinant Rabbit Monoclonal Antibody [SA43-06]
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概述
产品名称
JNK1+JNK2+JNK3 Recombinant Rabbit Monoclonal Antibody [SA43-06]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human JNK1 aa 1-423 / 427.
种属反应性
Human, Mouse, Rat, Cow, Monkey, Zebrafish
验证应用
WB, IF-Cell, IF-Tissue, IHC-P, IP
分子量
Predicted band size: 48/53 kDa
阳性对照
HeLa cell lysate, HEK-293 cell lysate, Jurkat cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, NIH/3T3, human breast cancer tissue, human brain tissue, mouse brain tissue, rat brain tissue.
偶联
unconjugated
克隆号
SA43-06
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IF-Cell
-
1:50
-
IF-Tissue
-
1:50
-
IHC-P
-
1:400-1:1,000
-
IP
-
Use at an assay dependent concentration.
发表文章中的应用
发表文章中的种属
靶点
功能
The c-Jun N-terminal kinases consist of ten isoforms derived from three genes: JNK1 (four isoforms), JNK2 (four isoforms) and JNK3 (two isoforms). Each gene is expressed as either 46 kDa or 55 kDa protein kinases, depending upon how the 3' coding region of the corresponding mRNA is processed. There have been no functional differences documented between the 46 kDa and the 55 kDa isoform, however, a second form of alternative splicing occurs within transcripts of JNK1 and JNK2, yielding JNK1-α, JNK2-α and JNK1-β and JNK2-β. Differences in interactions with protein substrates arise because of the mutually exclusive utilization of two exons within the kinase domain. and JNK2 are found in all cells and tissues. JNK3 is found mainly in the brain, but is also found in the heart and the testes.
背景文献
1. Cantrell, M. et al. 2015. c-Jun N-terminal kinase 2 prevents luminal cell commitment in normal mammary glands and tumors by inhibiting p53/Notch1 and breast cancer gene 1 expression. Oncotarget. 6: 11863-11881.
2. Marampon, F. et al. 2015. Vitamin D protects endothelial cells from irradiation-induced senescence and apoptosis by modulating MAPK/SirT1 axis. Journal of endocrinological investigation.
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
翻译后修饰
Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2. May be phosphorylated at Thr-183 and Tyr-185 by MAP3K1/MEKK1. Phosphorylated form is more concentrated at synapses than none-phosphorylated (By similarity).
亚细胞定位
Cytoplasm, Nucleus, Membrane, Mitochondrion
UNIPROT #
别名
C Jun kinase 2 antibody
c Jun N terminal kinase 1 antibody
c Jun N terminal kinase 2 antibody
c Jun N terminal kinase 3 antibody
c-Jun N-terminal kinase 1 antibody
JNK 46 antibody
JNK 55 antibody
JNK antibody
JNK-46 antibody
JNK1 antibody
展开C Jun kinase 2 antibody
c Jun N terminal kinase 1 antibody
c Jun N terminal kinase 2 antibody
c Jun N terminal kinase 3 antibody
c-Jun N-terminal kinase 1 antibody
JNK 46 antibody
JNK 55 antibody
JNK antibody
JNK-46 antibody
JNK1 antibody
JNK1A2 antibody
JNK2 antibody
JNK21B1/2 antibody
JNK2A antibody
JNK2ALPHA antibody
JNK2B antibody
JNK2BETA antibody
JNK3 alpha protein kinase antibody
JNK3 antibody
JNK3A antibody
Jun kinase antibody
JUN N terminal kinase antibody
MAP kinase 10 antibody
MAP kinase 8 antibody
MAP kinase 9 antibody
MAP kinase p49 3F12 antibody
MAPK 10 antibody
MAPK 8 antibody
MAPK 9 antibody
MAPK10 antibody
mapk8 antibody
MAPK9 antibody
Mitogen activated protein kinase 10 antibody
Mitogen activated protein kinase 8 antibody
Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody
Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody
Mitogen activated protein kinase 9 antibody
Mitogen-activated protein kinase 8 antibody
MK08_HUMAN antibody
p493F12 antibody
p54a antibody
p54aSAPK antibody
p54bSAPK antibody
PRKM10 antibody
PRKM8 antibody
PRKM9 antibody
SAPK antibody
SAPK(beta) antibody
SAPK1 antibody
SAPK1a antibody
SAPK1b antibody
SAPK1c antibody
Stress activated protein kinase 1 antibody
Stress activated protein kinase 1a antibody
Stress activated protein kinase 1b antibody
Stress activated protein kinase 1c antibody
Stress activated protein kinase beta antibody
Stress activated protein kinase JNK1 antibody
Stress activated protein kinase JNK2 antibody
Stress activated protein kinase JNK3 antibody
Stress-activated protein kinase 1c antibody
Stress-activated protein kinase JNK1 antibody
折叠图片
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Western blot analysis of JNK1+JNK2+JNK3 on different lysates with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: MCF7 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 48/53 kDa
Observed band size: 48/53 kDa
Exposure time: 35 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-28) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
ICC staining of JNK1+JNK2+JNK3 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-28) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-28) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-28) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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