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CH25H Rabbit Polyclonal Antibody

RMB: 1500

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Catalog# ER1919-26

CH25H Rabbit Polyclonal Antibody

Application

  • ELISA

  • IHC-P

  • IF

  • ICC

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

CH25H Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within Human CH25H aa 181-272.

种属反应性

Human

验证应用

ELISA, IHC-P, IF, ICC

分子量

32 kDa

阳性对照

Highest expression level in substantia nigra.

偶联

unconjugated

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • ELISA

  • 1:5000-10000

  • IHC-P

  • 1:100-500

  • IF

  • 1:100-500

  • ICC

  • 1:100-500

发表文章中的应用

WB See 1 publications below

发表文章中的种属

Human See 1 publications below

靶点

功能

CH25H (Cholesterol 25-hydroxylase), also known as h25OH, is a 272 amino acid endoplasmic membrane protein that belongs to the sterol desaturase family. CH25H contains clusters of histidine residues essential for catalytic activity and is involved in cholesterol and lipid metabolism. CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol leading to the repression of cholesterol biosynthetic enzymes. CH25H regulates lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H utilizes diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.

背景文献

1. Lund E.G.et al.cDNA cloning of mouse and human cholesterol 25-hydroxylases, polytopic membrane proteins that synthesize a potent oxysterol regulator of lipid metabolism.J. Biol. Chem. 273:34316-34327(1998)

亚细胞定位

Cytoplasm, cell membrane

UNIPROT #

SwissProt: O95992 Human

SwissProt: Q9Z0F5 Mouse

SwissProt: Q4QQV7 Rat

别名

C25H antibody

Ch25h antibody

CH25H_HUMAN antibody

Cholesterol 25 hydroxylase antibody

Cholesterol 25 monooxygenase antibody

Cholesterol 25-hydroxylase antibody

Cholesterol 25-monooxygenase antibody

EC 1.14.99.38 antibody

EC=1.14.99.38 antibody

h25OH antibody

图片

  • Immunohistochemical analysis of paraffin-embedded human brain glioma using anti-CH25H antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER1919-26" style="font-weight: bold;text-decoration: underline;">ER1919-26</a>, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain glioma using anti-CH25H antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1919-26, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
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