ADAMTS5 Rabbit Polyclonal Antibody
Catalog# ER1903-32
ADAMTS5 Rabbit Polyclonal Antibody
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WB
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ELISA
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IHC-P
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Human
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Mouse
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Rat
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Dog
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Pig
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Cow
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Horse
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Rabbit
概述
产品名称
ADAMTS5 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human Aggrecan 2 511-610/930
种属反应性
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit
验证应用
WB, ELISA, IHC-P
分子量
102 KDa
阳性对照
Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient.
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-2000
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ELISA
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1:5000-10000
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IHC-P
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1:100-500
发表文章中的应用
发表文章中的种属
| Mouse | See 2 publications below |
靶点
功能
Metalloproteinase that plays an important role in connective tissue organization, development, inflammation, arthritis, and cell migration. ADAMTS5 is an extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including aggrecan, versican, brevican and neurocan (PubMed:16133547, PubMed:18992360). Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its versican remodeling properties. Participates in development of brown adipose tissue and browning of white adipose tissue. Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection.
背景文献
暂无
亚细胞定位
Secreted, extracellular space, extracellular matrix.
UNIPROT #
别名
A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody
A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody
A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 antibody
A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif
5 (aggrecanase 2) antibody
A disintegrin-like and metalloprotease with thrombospondin type 1 motif
5 antibody
A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 antibody
ADAM metallopeptidase with thrombospondin type 1 motif 5 antibody
ADAM TS 11 antibody
展开图片
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Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Aggrecanase-2/ADAMTS5 Polyclonal Antibody, Unconjugated (ER1903-32) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining -
Sample:Liver (Mouse) Lysate at 40 ug
Primary: Anti-ADAMTS5 (ER1903-32) at 1/300 dilution
Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 102 kD
Observed band size: 102 kD
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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PD184352 exerts anti-inflammatory and antioxidant effects by promoting activation of the Nrf2/HO-1 axis
Author:
PMID: 37028460
应用: WB
反应种属: Mouse
发表时间: 2023 May
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Citation
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7,8-Dihydroxyflavone modulates bone formation and resorption and ameliorates ovariectomy-induced osteoporosis. eLife, 10, e64872.
Author: Xue, F., Zhao, Z., Gu, Y., Han, J., Ye, K., & Zhang, Y.
PMID: 34227467
应用: WB
反应种属: Mouse
发表时间: 2021 Jul
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Citation
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