Hip1 Mouse Monoclonal Antibody [13E1]
Catalog# EM1901-04
Hip1 Mouse Monoclonal Antibody [13E1]
-
WB
-
IF-Cell
-
IHC-P
-
Human
-
Mouse
-
Rat
概述
产品名称
Hip1 Mouse Monoclonal Antibody [13E1]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within Human Hip1 aa 1-50 / 1,029.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IHC-P
分子量
Predicted band size: 116 kDa
阳性对照
HCT 116 cell lysates, SH-SY5Y, rat brain tissue, human colon cancer tissue, human prostate cancer tissue, mouse brain tissue.
偶联
unconjugated
克隆号
13E1
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:500-1:2,000
-
IF-Cell
-
1:50-1:200
-
IHC-P
-
1:50-1:200
靶点
功能
Huntington disease is associated with the expansion of a polyglutamine tract, greater than 35 repeats, in the HD gene product huntingtin. HIP1 (huntingtin-interacting protein 1), a membrane-associated protein, binds specifically to the N-terminus of human huntingtin. HIP1 is ubiquitously expressed in different brain regions at low levels, and exhibits nearly identical subcellular fractionation as huntingtin. The huntingtin-HIP1 interaction is restricted to the brain and is inversely correlated to the polyglutamine length in the huntingtin, suggesting that loss of normal huntingtin-HIP1 interaction may compromise the membrane-cytoskeletal integrity in the brain. HIP1 contains an endocytic multidomain protein with a C-terminal Actin-binding domain, a central coiled-coil forming region and an N-terminal ENTH domain. HIP1 may be involved in vesicle trafficking; the structural integrity of HIP1 is crucial for maintenance of normal vesicle size in vivo. HIP12 is a non-proapoptotic member of the HIP gene family that is expressed in the brain and shares a similar subcellular distribution pattern with HIP1. However, HIP12 differs from HIP1 in its pattern of expression at both the mRNA and protein level. HIP12 does not directly interact with huntingtin but can interact with HIP1.
背景文献
1. Wanker EE et al. HIP-I: a huntingtin interacting protein isolated by the yeast two-hybrid system. Hum Mol Genet 6:487-495 (1997).
2. Hackam AS et al. Huntingtin interacting protein 1 induces apoptosis via a novel caspase-dependent death effector domain. J Biol Chem 275:41299-41308 (2000).
序列相似性
Belongs to the SLA2 family.
组织特异性
Ubiquitously expressed with the highest level in brain. Expression is up-regulated in prostate and colon cancer.
亚细胞定位
Nucleus. Cytoplasm.
别名
2610109B09Rik antibody
A930014B11Rik antibody
E130315I21Rik antibody
HIP 1 antibody
HIP I antibody
HIP-1 antibody
HIP-I antibody
hip1 antibody
HIP1/PDGFRB fusion gene antibody
HIP1/PDGFRB fusion gene, included antibody
展开图片
-
Western blot analysis of Hip1 on HCT 116 cell lysates with Mouse anti-Hip1 antibody (EM1901-04) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 116 kDa
Observed band size: 116 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining of Hip1 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-04, 1/50 dilution) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
-
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Hip1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-04, 1/200 dilution) for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Hip1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with primary antibody (EM1901-04, 1/200 dilution) for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Hip1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with primary antibody (EM1901-04, 1/200 dilution) for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hip1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with primary antibody (EM1901-04, 1/200 dilution) for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"