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CD68 Rabbit Polyclonal Antibody

RMB: 1500

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Catalog# ER1901-32

CD68 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • ICC

  • IF

  • ELISA

Reactivity

  • Human

  • Mouse

  • Cow

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

CD68 Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within Human CD68 aa 25-74 / 354.

种属反应性

Human, Mouse, Cow

验证应用

WB, IHC-P, ICC, IF, ELISA

分子量

Predicted band size: 37 kDa

偶联

unconjugated

RRID

AB_3069325

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:100-1:500

  • ICC

  • 1:100

  • IF

  • : 1:100-1:500

  • ELISA

  • 1:5,000-10,000

发表文章中的应用

IF See 1 publications below

发表文章中的种属

Human See 1 publications below

靶点

功能

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms.

背景文献

1. Chistiakov DA. et. al. CD68/macrosialin: not just a histochemical marker. Lab Invest. 2017 Jan;97(1):4-13.

2. Tochio H. et. al. CD68-Positive Cells in Hepatic Angiomyolipoma. Oncology. 2017;92 Suppl 1:35-39.

序列相似性

Belongs to the LAMP family.

组织特异性

Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.

翻译后修饰

N- and O-glycosylated.

亚细胞定位

Cell membrane, endosome membrane, lysosome membrane.

UNIPROT #

SwissProt: P34810 Human

SwissProt: P31996 Mouse

别名

CD 68 antibody

CD68 antibody

CD68 antigen antibody

CD68 molecule antibody

CD68_HUMAN antibody

DKFZp686M18236 antibody

gp11 antibody

Gp110 antibody

LAMP4 antibody

Macrophage antigen CD68 (microsialin) antibody

展开

图片

  • This image was generously provided by Amanda Brown, Ph.D. from Johns Hopkins University School of Medicine. Paraffin embedded human brain tissue labeled with Rabbit Anti-CD68 Polyclonal Antibody, Unconjugated (<a href="/products/ER1901-32" style="font-weight: bold;text-decoration: underline;">ER1901-32</a>) at 1:50 followed by conjugation to the secondary antibody and Permanent Fast Red Quanto staining

    This image was generously provided by Amanda Brown, Ph.D. from Johns Hopkins University School of Medicine. Paraffin embedded human brain tissue labeled with Rabbit Anti-CD68 Polyclonal Antibody, Unconjugated (ER1901-32) at 1:50 followed by conjugation to the secondary antibody and Permanent Fast Red Quanto staining

  • Tissue/cell: human mammary cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;<br />Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;<br />Incubation: Anti-CD68 Polyclonal Antibody, Unconjugated(<a href="/products/ER1901-32" style="font-weight: bold;text-decoration: underline;">ER1901-32</a>) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Tissue/cell: human mammary cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-CD68 Polyclonal Antibody, Unconjugated(ER1901-32) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

  • Sample:<br />Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug<br />Lane 2: Lymph node (Rat) Cell Lysate at 30 ug<br />Primary: Anti-CD68 (<a href="/products/ER1901-32" style="font-weight: bold;text-decoration: underline;">ER1901-32</a>) at 1/1000 dilution<br />Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20,000 dilution<br />Predicted band size: 110/185-200 kD<br />Observed band size: 110 kD

    Sample:
    Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug
    Lane 2: Lymph node (Rat) Cell Lysate at 30 ug
    Primary: Anti-CD68 (ER1901-32) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20,000 dilution
    Predicted band size: 110/185-200 kD
    Observed band size: 110 kD

  • Sample:<br />Lane 1: A431 (Human) Cell Lysate at 30 ug<br />Lane 2: A549 (Human) Cell Lysate at 30 ug<br />Lane 3: Jurkat (Human) Cell Lysate at 30 ug<br />Lane 4: Huvec (Human) Cell Lysate at 30 ug<br />Primary: Anti-CD68 (<a href="/products/ER1901-32" style="font-weight: bold;text-decoration: underline;">ER1901-32</a>) at 1/500 dilution<br />Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20,000 dilution<br />Predicted band size: 110/185-200 kD<br />Observed band size: 110 kD

    Sample:
    Lane 1: A431 (Human) Cell Lysate at 30 ug
    Lane 2: A549 (Human) Cell Lysate at 30 ug
    Lane 3: Jurkat (Human) Cell Lysate at 30 ug
    Lane 4: Huvec (Human) Cell Lysate at 30 ug
    Primary: Anti-CD68 (ER1901-32) at 1/500 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20,000 dilution
    Predicted band size: 110/185-200 kD
    Observed band size: 110 kD

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. IF

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