CD68 Mouse Monoclonal Antibody [F3-D7]
Catalog# EM1706-11
CD68 Mouse Monoclonal Antibody [F3-D7]
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WB
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IHC-P
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ICC
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FC
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Human
概述
产品名称
CD68 Mouse Monoclonal Antibody [F3-D7]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein
种属反应性
Human
验证应用
WB, IHC-P, ICC, FC
分子量
37 kDa
阳性对照
Human CD68 recombinant protein, CD68-hIgGFc transfected HEK293 cell lysate, U937, Hela, HepG2, human endometrial cancer tissue, human bladder cancer tissue, Jurkat.
偶联
unconjugated
克隆号
F3-D7
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
4℃; -20℃ for long term storage.
存储缓冲液
Purified antibody in PBS with 0.05% sodium azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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ICC
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1:200-1:1,000
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IHC-P
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1:200-1:1,000
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FC
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1:50-1:100
发表文章中的应用
发表文章中的种属
| Human | See 2 publications below |
| Rat | See 1 publications below |
| Mouse | See 1 publications below |
靶点
功能
CD68, which is homologous to the mouse antigen macrosialin, belongs to a family of acidic, highly glycosylated lysosomal glycoproteins (LGPs) that includes lamp-1 and lamp-2. CD68 is found in cytoplasmic granules and in the cytoplasm of various non-hematopoietic tissues including liver and kidney tubules and glomeruli. CD68 is also found, to a lesser extent, on the surface of macrophages, monocytes, neutrophils, basophils and large lymphocytes. LGPs are major components of lysosomal membranes and may act to protect the membranes from attack by hydrolases.
背景文献
1. Cao S et al. Hydrogen sulfide attenuates brain edema in early brain injury after subarachnoid hemorrhage in rats: Possible involvement of MMP-9 induced blood-brain barrier disruption and AQP4 expression. Neurosci Lett 621:88-97 (2016).
2. Qi X et al. Development of inCVAX, In situ Cancer Vaccine, and Its Immune Response in Mice with Hepatocellular Cancer. J Clin Cell Immunol 7:N/A (2016).
亚细胞定位
Cell membrane and Endosome membrane. Lysosome membrane.
UNIPROT #
别名
CD 68 antibody
CD68 antibody
CD68 antigen antibody
CD68 molecule antibody
CD68_HUMAN antibody
DKFZp686M18236 antibody
gp11 antibody
Gp110 antibody
LAMP4 antibody
Macrophage antigen CD68 (microsialin) antibody
展开图片
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Western blot analysis of CD68 on human CD68 recombinant protein using anti-CD68 antibody at 1/1,000 dilution.
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Western blot analysis of CD68 on HEK293 (1) and CD68-hIgGFc transfected HEK293 (2) cell lysate using anti-CD68 antibody at 1/1,000 dilution.
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Western blot analysis of CD68 on different cell lysate using anti-CD68 antibody at 1/1,000 dilution.
Positive control: Line1: U937 Line1: Hela Line2: HepG2 Line3: Jurkat -
ICC staining CD68 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-CD68 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CD68 antibody. Counter stained with hematoxylin.
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Flow cytometric analysis of Hela cells with CD68 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Crosstalk between cancer-associated fibroblasts and myeloid cells shape the heterogeneous microenvironment of gastric cancer
Author: Peng Zhiwei,et al
PMID: NO pmid20240701
应用: IF,mIHC
反应种属: Human
发表时间: 2024 Jul
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Citation
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Irisin attenuates acute lung injury by suppressing the pyroptosis of alveolar macrophages
Author: Han, Z., Ma, J., Han, Y., Yuan, G., Jiao, R., & Meng, A.
PMID: 36896789
应用: IF
反应种属: Mouse
发表时间: 2023 Apr
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Citation
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Microglial responses and pain behaviors are exacerbated by chronic sleep deprivation in rats with chronic pain via neuroinflammatory pathways
Author:
PMID: 36096338
应用: IF-tissue
反应种属: Rat
发表时间: 2022 Nov
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Citation
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Pan-cancer analyses demonstrate that ANKRD6 is associated with a poor prognosis and correlates with M2 macrophage infiltration in colon cancer. Chinese journal of cancer research = Chung-kuo yen cheng yen chiu, 33(1), 93–102.
Author: Bai, R., Wu, D., Shi, Z., Hu, W., Li, J., Chen, Y., Ge, W., Yuan, Y., & Zheng, S.
PMID: 33707932
应用: IHC
反应种属: Human
发表时间: 2021 Feb
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Citation
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