CD45 Rabbit Polyclonal Antibody
Catalog# ER1905-93
CD45 Rabbit Polyclonal Antibody
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WB
-
ELISA
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IHC-P
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FC
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IF
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ICC
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Human
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Mouse
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Rat
概述
产品名称
CD45 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from rat CD45/B220 501-600/1273
种属反应性
Human, Mouse, Rat
验证应用
WB, ELISA, IHC-P, FC, IF, ICC
分子量
137 KDa
阳性对照
Raji cell, Uterus (Mouse) Lysate, MCF-7 (Human) Lysate, Molt4 cell, Jurkat cell.
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-2000
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ELISA
-
1:5000-10000
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IHC-P
-
1:100-500
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FC
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1μg/Test
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IF
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1:50-200
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ICC
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1:100-500
靶点
功能
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
背景文献
暂无
亚细胞定位
Membrane; Single-pass type I membrane protein. Membrane raft. Note=Colocalized with DPP4 in membrane rafts.
UNIPROT #
别名
B220 antibody
CD 45 antibody
CD45 antibody
CD45 antigen antibody
CD45R antibody
GP180 antibody
L-CA antibody
LCA antibody
Leukocyte common antigen antibody
loc antibody
展开图片
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Blank control: Raji(blue).
Primary Antibody:Rabbit Anti- CD45 antibody(ER1905-93), Dilution: 1μg in 100 1μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. -
Sample:
Uterus (Mouse) Lysate at 40 ug
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-CD45 (ER1905-93) at 1/300 dilution
Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 137 kD
Observed band size: 130 kD -
Sample:
MCF-7 (Human) Lysate at 40 ug
Primary: Anti-CD45 (ER1905-93) at 1/300 dilution
Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 137 kD
Observed band size: 130 kD -
Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti-CD45 antibody (ER1905-93)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. -
Blank control: Jurkat.
Primary Antibody (green line): Rabbit Anti-CD45 antibody (ER1905-93)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. -
Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti-CD45/PTPRC/FITC Conjugated antibody (ER1905-93-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点&同通路的产品
CD45 Rabbit Polyclonal Antibody
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
CD45 Rabbit Polyclonal Antibody
Application: WB,FC
Reactivity: Human
Conjugate: unconjugated
