CD45 Rabbit Polyclonal Antibody
Catalog# ER1902-02
CD45 Rabbit Polyclonal Antibody
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WB
-
FC
-
Human
概述
产品名称
CD45 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human CD45 aa 1-60.
种属反应性
Human
验证应用
WB, FC
分子量
Predicted band size 147 kDa
阳性对照
U937 cell lysates, Jurkat cell lysates, Jurkat.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:500-1:1,000
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FC
-
1:50-1:100
靶点
功能
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
背景文献
1. Rheinländer A. et. al. CD45 in human physiology and clinical medicine. Immunol Lett. 2018 Apr;196:22-32.
2. Fogle JE. et. al. CD45+ and CD45- lymphocyte populations identified by flow cytometry from dogs with lymphoma exhibit similar morphology and the same clonal (B cell or T cell) lineage. Vet Immunol Immunopathol. 2015 Dec 15;168(3-4):242-8.
序列相似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
组织特异性
Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes.
翻译后修饰
Heavily N- and O-glycosylated.
亚细胞定位
Cell membrane, membrane raft.
UNIPROT #
别名
B220 antibody
CD 45 antibody
CD45 antibody
CD45 antigen antibody
CD45R antibody
GP180 antibody
L-CA antibody
LCA antibody
Leukocyte common antigen antibody
loc antibody
展开图片
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Western blot analysis of CD45 on U937 cell lysates using anti-CD45 antibody.
Lane 1: Anti-CD45 Antibody (1/500).
Lane 2: Anti-CD45 Antibody, pre-incubated with the immunizaiton peptide. -
Western blot analysis of CD45 on Jurkat cell lysates using anti-CD45 antibody.
Lane 1: Anti-CD45 Antibody (1/500).
Lane 2: Anti-CD45 Antibody, pre-incubated with the immunizaiton peptide. -
Flow cytometric analysis of CD45 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
CD45 Recombinant Rabbit Monoclonal Antibody [JE03-05]
Application: WB,IHC-P,FC,IF-Tissue,mIHC
Reactivity: Human
Conjugate: unconjugated
同靶点&同通路的产品
CD45 Rabbit Polyclonal Antibody
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated