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CD34 Rabbit Polyclonal Antibody

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Catalog# 0108-2

CD34 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Zebrafish

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

CD34 Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within human CD34 aa 336-385.

种属反应性

Human, Mouse, Zebrafish

验证应用

WB, IF-Cell, IHC-P

分子量

Predicted band size: 41 kDa

阳性对照

Human brain, mouse brain, mouse testis, Mouse thymus, mouse spleen, Jurkat, TF-1, F9, human embryonic stem cell, human tonsil tissue

偶联

unconjugated

RRID

AB_3096758

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:3,000

发表文章中的应用

IHC See 1 publications below
WB See 1 publications below

发表文章中的种属

Mouse See 2 publications below
Human See 1 publications below

靶点

功能

CD34 is a transmembrane phosphoglycoprotein protein encoded by the CD34 gene in humans, mice, rats and other species. The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early haematopoietic and vascular-associated progenitor cells. However, little is known about its exact function. CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. It is expressed on lymph node endothelia, whereas the L-selectin to which it binds is on the T cell. Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. Finally, recent data suggest CD34 may also play a more selective role in chemokine-dependent migration of eosinophils and dendritic cell precursors. Regardless of its mode of action, under all circumstances CD34, and its relatives podocalyxin and endoglycan, facilitates cell migration.

背景文献

1. Freund D., Wiebe G.J., Ehninger G., Corbeil D.; "Sequence analysis of the human CD34 antigen."; Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.

2. The MGC Project Team;"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

3. Sutherland D.R., Watt S.M., Dowden G., Karhi K., Baker M.A., Greaves M.F., Smart J.E.;"Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34.";Leukemia 2:793-803(1988).

序列相似性

Belongs to the CD34 family.

组织特异性

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

翻译后修饰

Highly glycosylated.; Phosphorylated on serine residues by PKC.

亚细胞定位

Cell membrane

UNIPROT #

SwissProt: P28906 Human

SwissProt: Q64314 Mouse

别名

CD34 antibody

CD34 antigen antibody

CD34 molecule antibody

CD34_HUMAN antibody

Cluster designation 34 antibody

Hematopoietic progenitor cell antigen CD34 antibody

HPCA1 antibody

Mucosialin antibody

OTTHUMP00000034733 antibody

OTTHUMP00000034734 antibody

图片

  • Western blot analysis of CD34 on different cell lysates using anti-CD34 antibody at 1/1,000 dilution.<br /><br />Positive control: <br />Lane 1: Human brain lysate<br />Lane 2: Mouse brain lysate<br />Lane 3: Mouse testis lysate<br />Lane 4: Mouse thymus lysate<br />Lane 5: Mouse spleen lysate<br />Lane 6:Jurkat lysate<br />Lane 7: TF-1 lysate<br />Lane 8: F9 lysate<br /><br />Predicted band size: 41 kDa<br />Observed band size: 90 kDa

    Western blot analysis of CD34 on different cell lysates using anti-CD34 antibody at 1/1,000 dilution.

    Positive control:
    Lane 1: Human brain lysate
    Lane 2: Mouse brain lysate
    Lane 3: Mouse testis lysate
    Lane 4: Mouse thymus lysate
    Lane 5: Mouse spleen lysate
    Lane 6:Jurkat lysate
    Lane 7: TF-1 lysate
    Lane 8: F9 lysate

    Predicted band size: 41 kDa
    Observed band size: 90 kDa

  • ICC staining of CD34 in hES cells (green). Formalin fixed cells were permeabilized with 0.25% Triton X100/PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/0108-2" style="font-weight: bold;text-decoration: underline;">0108-2</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.

    ICC staining of CD34 in hES cells (green). Formalin fixed cells were permeabilized with 0.25% Triton X100/PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (0108-2, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/0108-2" style="font-weight: bold;text-decoration: underline;">0108-2</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0108-2, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. IHC
  2. WB

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