您的当前位置: 首页 > 产品 > 产品详情 > EM40507

Anti-AKT1 Mouse Monoclonal Antibody [D9-9-C9]

小鼠单抗 | 货号: EM40507


规格:

价格:RMB   1500
现在订购,【现货】
相关产品:

暂无,点击查看热门产品

  • 概述

  • 产品描述 The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mice lacking Akt1 display a 25% reduction in body mass, indicating that Akt1 is critical for transmitting growth-promoting signals, most likely via the igf1 receptor. Mice lacking Akt1 are also resistant to cancer: They experience considerable delay in tumor growth initiated by the large T antigen or the Neuoncogene.
  • 产品名称 Anti-AKT1 Mouse Monoclonal Antibody [D9-9-C9]
  • 分子量 Predicted band size: 56 kDa
  • 种属反应性 Human,Mouse,Rat
  • 验证应用 WB,IF-Cell,IHC-P,FC
  • 抗体类型 小鼠单抗
  • 免疫原 Synthetic peptide within human AKT1 aa 1-80.
  • 偶联 Non-conjugated
  • RRID AB_3068997
  • 性能

  • 形态 Liquid
  • 浓度 2 mg/mL.
  • 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • 存储缓冲液 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • 亚型 IgG2b
  • 纯化方式 Protein A affinity purified.
  • 亚细胞定位 Nucleus, cytoplasm, cell membrane.
  • 数据链接 SwissProt: P31749 Human
    SwissProt: P31750 Mouse
    SwissProt: P47196 Rat
  • 其它名称
    • AKT 1 antibody
    • AKT antibody
    • AKT1 antibody
  • 应用

    WB: 1:2,000
    IHC-P: 1:100-1:200
    IF-Cell: 1:100-1:200
    FC: 1:100-1:200

  • 背景文献

  • 1. "PIKE-A is amplified in human cancers and prevents apoptosis by up-regulating Akt." Ahn J.-Y., Hu Y., Kroll T.G., Allard P., Ye K. Proc. Natl. Acad. Sci. U.S.A. 101:6993-6998(2004)

    2. "Akt/PKB regulates actin organization and cell motility via Girdin/APE." Enomoto A., Murakami H., Asai N., Morone N., Watanabe T., Kawai K., Murakumo Y., Usukura J., Kaibuchi K., Takahashi M. Dev. Cell 9:389-402(2005)

    3. "The pro-apoptotic kinase Mst1 and its caspase cleavage products are direct inhibitors of Akt1." Cinar B., Fang P.K., Lutchman M., Di Vizio D., Adam R.M., Pavlova N., Rubin M.A., Yelick P.C., Freeman M.R. EMBO J. 26:4523-4534(2007)

    4. "The actin-bundling protein palladin is an Akt1-specific substrate that regulates breast cancer cell migration." Chin Y.R., Toker A. Mol. Cell 38:333-344(2010)
  • Publishing research using Anti-AKT1 Mouse Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

  • PMID:
    35242756
    Protective Effect of Grape Seed Proanthocyanidins on Oxidative Damage of Chicken Follicular Granulosa Cells by Inhibiting FoxO1-Mediated Autophagy Author: Zhou, S., Zhao, A., Wu, Y., Mi, Y., & Zhang, C. Published: 2022 Application: WB Species: Hen Journal: Frontiers in Cell and Developmental Biology IF: 6.684
  • PMID:
    30488492
    Biogenic nanoselenium particles activate Nrf2-ARE pathway by phosphorylating p38,ERK1/2,and AKT on IPEC-J2 cells. Author: Yizhen Wang Published: 2019 Application: WB Species: The porcine jejunum epithelial (IPEC‐J2) cells Journal: J Cell Physiol. IF: 4.522

Choose your product,application and species below,then click "Submit".

On the next page you can complete and submit your review.

  • * Code No.
  • *Application
  • *Sample
  • Species Tested
  • Against
  • Choose text
  • Content

Leave us a review

Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols