TBR2 Recombinant Rabbit Monoclonal Antibody [PSH07-88]
Catalog# HA722918
TBR2 Recombinant Rabbit Monoclonal Antibody [PSH07-88]
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WB
-
IHC-P
-
IHC-Fr
-
Human
-
Mouse
概述
产品名称
TBR2 Recombinant Rabbit Monoclonal Antibody [PSH07-88]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human TBR2 aa 1-300.
种属反应性
Human, Mouse
验证应用
WB, IHC-P, IHC-Fr
分子量
Predicted band size: 73 kDa
阳性对照
NK-92 cell lysate, Mouse embryo tissue lysate, E14.5 mouse embryonic brain tissue.
偶联
unconjugated
克隆号
PSH07-88
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IHC-P
-
1:5,000
-
IHC-Fr
-
1:500
靶点
功能
Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.
背景文献
1. Joulia E et al. Eomes-dependent mitochondrial regulation promotes survival of pathogenic CD4+ T cells during inflammation. J Exp Med. 2024 Feb.
2. Wong P et al. T-BET and EOMES sustain mature human NK cell identity and antitumor function. J Clin Invest. 2023 Jul.
亚细胞定位
Nucleus.
别名
eomes
EOMES_HUMAN
Eomesodermin
Eomesodermin homolog
t box brain 2
T-box brain protein 2
T-brain-2
Tbr 2
TBR-2
图片
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Immunofluorescence analysis of frozen E14.5 mouse embryonic brain tissue with Rabbit anti-TBR2 antibody (HA722918) at 1/500 dilution.
The section was not undergone antigen retrieval.
The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722918, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunohistochemical analysis of paraffin-embedded E14.5 mouse embryonic brain tissue with Rabbit anti-TBR2 antibody (HA722918) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722918) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of TBR2 on different lysates with Rabbit anti-TBR2 antibody (HA722918) at 1/1,000 dilution.
Lane 1: NK-92 cell lysate
Lane 2: HeLa cell lysate (negative)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 73 kDa
Observed band size: 73/85 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722918) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of TBR2 on mouse embryo tissue lysates with Rabbit anti-TBR2 antibody (HA722918) at 1/1,000 dilution.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 73 kDa
Observed band size: 73/85 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722918) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"