Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [PSH07-76]
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11+
Catalog# HA722892
Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [PSH07-76]
-
WB
-
IHC-P
-
IF-Tissue
-
IHC-Fr
-
IF-Cell
-
IP
-
mIHC
-
Mouse
-
Rat
概述
产品名称
Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [PSH07-76]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse Mannose Receptor(CD206) aa 1-1,409.
种属反应性
Mouse, Rat
验证应用
WB, IHC-P, IF-Tissue, IHC-Fr, IF-Cell, IP, mIHC
分子量
Predicted band size: 165 kDa
阳性对照
RAW264.7 treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours cell lysate, Mouse lung tissue lysate, Mouse spleen tissue lysate, Rat brain tissue lysate, Rat lung tissue lysate, Rat liver tissue lysate, mouse spleen tissue, mouse liver tissue, rat liver tissue, mouse osteosarcoma tissue.
偶联
unconjugated
克隆号
PSH07-76
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:2,000
-
IHC-P
-
1:1,000
-
IF-Tissue
-
1:500
-
IHC-Fr
-
1:500-1:1,000
-
IF-Cell
-
1:50
-
IP
-
1-2μg/sample
-
mIHC
-
1:2,000
靶点
功能
The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages, immature dendritic cells and liver sinusoidal endothelial cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205). The receptor recognises terminal mannose, N-acetylglucosamine and fucose residues on glycans attached to proteins found on the surface of some microorganisms, playing a role in both the innate and adaptive immune systems. Additional functions include clearance of glycoproteins from circulation, including sulphated glycoprotein hormones and glycoproteins released in response to pathological events. The mannose receptor recycles continuously between the plasma membrane and endosomal compartments in a clathrin-dependent manner.
背景文献
1. Nielsen MC et al. Macrophage Activation Markers, CD163 and CD206, in Acute-on-Chronic Liver Failure. Cells. 2020 May
2. Nawaz A et al. Depletion of CD206(+) M2-like macrophages induces fibro-adipogenic progenitors activation and muscle regeneration. Nat Commun. 2022 Nov
亚细胞定位
Endosome membrane, Cell membrane.
别名
bA541I19.1 antibody
C type lectin domain family 13 member D antibody
C-type lectin domain family 13 member D antibody
CD 206 antibody
CD206 antibody
CD206 antigen antibody
CLEC13D antibody
CLEC13DL antibody
Macrophage mannose receptor 1 antibody
Macrophage mannose receptor 1 like protein 1 antibody
展开图片
-
☑ Cell treatment (CT)
Western blot analysis of Mannose Receptor(CD206) on different lysates with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/2,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours cell lysate (Macrophage M2 polarization, positive)
Lane 3: RAW264.7 cell lysate
Lane 4: RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate (Macrophage M1 polarization, negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 165 kDa
Observed band size: 200 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722892) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Mannose Receptor(CD206) on different lysates with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/1,000 dilution.
Lane 1: Mouse lung tissue lysate (20 µg/Lane)
Lane 2: Mouse spleen tissue lysate (20 µg/Lane)
Lane 3: Rat brain tissue lysate (20 µg/Lane)
Lane 4: Rat lung tissue lysate (20 µg/Lane)
Lane 5: Rat liver tissue lysate (20 µg/Lane)
Predicted band size: 165 kDa
Observed band size: 200 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722892) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722892) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722892) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722892) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of RAW264.7 cells treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours (Macrophage M2 polarization, positive) labeling Mannose Receptor(CD206) with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of RAW264.7 cells treated with 0.1μg/mL LPS for 6 hours (Macrophage M1 polarization, negative) labeling Mannose Receptor(CD206) with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunofluorescence analysis of paraffin-embedded mouse spleen tissue labeling Mannose Receptor(CD206) with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722892, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded mouse liver tissue labeling Mannose Receptor(CD206) with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722892, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of frozen mouse spleen tissue with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/1,000 dilution.
The section was not undergone antigen retrieval.
The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722892, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of frozen mouse liver tissue with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/500 dilution.
The section was not undergone antigen retrieval.
The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722892, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Mannose Receptor(CD206) was immunoprecipitated from 0.2 mg rat lung tissue lysate with HA722892 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722892 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: rat lung tissue lysate (input)
Lane 2: HA722892 IP in rat lung tissue lysate
Lane 3: Rabbit IgG instead of HA722892 in rat lung tissue lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute; ECL: K1801 -
mIHC analysis of mouse liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/2,000 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
-
mIHC analysis of mouse osteosarcoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/2,000 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
-
mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/2,000 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"