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VGLUT2 Recombinant Antibody [PSH10-54] - Rat IgG1 (Chimeric)

RMB: 699 特惠 1500

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Catalog# HA601393

VGLUT2 Recombinant Antibody [PSH10-54] - Rat IgG1 (Chimeric)

Application

  • IHC-Fr

  • WB

Reactivity

  • Mouse

  • Rat

概述

产品名称

VGLUT2 Recombinant Antibody [PSH10-54] - Rat IgG1 (Chimeric)

抗体类型

Recombinant Chimeric Antibody

种属反应性

Mouse, Rat

验证应用

IHC-Fr, WB

分子量

Predicted band size: 65 kDa

阳性对照

Mouse cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

偶联

unconjugated

克隆号

PSH10-54

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG1

纯化方式

Protein A affinity purified.

应用稀释度

  • IHC-Fr

  • 1:500

  • WB

  • 1:2,000

靶点

功能

Predicted to enable L-glutamate transmembrane transporter activity and neurotransmitter transmembrane transporter activity. Involved in neurotransmitter loading into synaptic vesicle. Predicted to be located in synaptic vesicle. Predicted to be active in excitatory synapse. Predicted to be integral component of synaptic vesicle membrane.

背景文献

1. Buck SA et al. Roles of VGLUT2 and Dopamine/Glutamate Co-Transmission in Selective Vulnerability to Dopamine Neurodegeneration. ACS Chem Neurosci. 2022 Jan

2. Zhang Y et al. VGluT2 neuron subtypes in the paraventricular thalamic nucleus regulate depression in paraquat-induced Parkinson\'s disease. J Hazard Mater. 2024 Jul

亚细胞定位

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Synapse, synaptosome, Cell membrane.

UNIPROT #

SwissProt: Q8BLE7 Mouse

SwissProt: Q9JI12 Rat

别名

Differentiation associated BNPI antibody

Differentiation associated Na dependent inorganic phosphate cotransporter antibody

Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody

Differentiation-associated BNPI antibody

Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody

DNPI antibody

SLC17A6 antibody

Sodium dependent inorganic phosphate cotransporter antibody

Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody

Solute carrier family 17 member 6 antibody

展开

图片

  • Immunofluorescence analysis of frozen mouse cerebellum tissue with Rat anti-VGLUT2 antibody (<a href="/products/HA601393" style="font-weight: bold;text-decoration: underline;">HA601393</a>) at 1/500 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span> <br /><br />The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA601393" style="font-weight: bold;text-decoration: underline;">HA601393</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen mouse cerebellum tissue with Rat anti-VGLUT2 antibody (HA601393) at 1/500 dilution.

    The section was not undergone antigen retrieval.

    The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601393, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunofluorescence analysis of frozen mouse cerebrum tissue with Rat anti-VGLUT2 antibody (<a href="/products/HA601393" style="font-weight: bold;text-decoration: underline;">HA601393</a>) at 1/500 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span> <br /><br />The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA601393" style="font-weight: bold;text-decoration: underline;">HA601393</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen mouse cerebrum tissue with Rat anti-VGLUT2 antibody (HA601393) at 1/500 dilution.

    The section was not undergone antigen retrieval.

    The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601393, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunofluorescence analysis of frozen rat cerebellum tissue with Rat anti-VGLUT2 antibody (<a href="/products/HA601393" style="font-weight: bold;text-decoration: underline;">HA601393</a>) at 1/500 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span> <br /><br />The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA601393" style="font-weight: bold;text-decoration: underline;">HA601393</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).<br /><br />Rabbit Anti-PSD95 (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>, red) was stained at 1/1,000 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunofluorescence analysis of frozen rat cerebellum tissue with Rat anti-VGLUT2 antibody (HA601393) at 1/500 dilution.

    The section was not undergone antigen retrieval.

    The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601393, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).

    Rabbit Anti-PSD95 (ET1602-20, red) was stained at 1/1,000 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of VGLUT2 on different lysates with Rat anti-VGLUT2 antibody (<a href="/products/HA601393" style="font-weight: bold;text-decoration: underline;">HA601393</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse lung tissue lysate (negative)<br />Lane 3: Rat brain tissue lysate<br />Lane 4: Rat lung tissue lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 65 kDa<br />Observed band size: 65 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601393" style="font-weight: bold;text-decoration: underline;">HA601393</a>) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of VGLUT2 on different lysates with Rat anti-VGLUT2 antibody (HA601393) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse lung tissue lysate (negative)
    Lane 3: Rat brain tissue lysate
    Lane 4: Rat lung tissue lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

    Exposure time: 4 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601393) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"