SOX17 Rabbit Polyclonal Antibody
Catalog# HA500476
SOX17 Rabbit Polyclonal Antibody
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WB
-
FC
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Human
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Mouse
-
Rat
概述
产品名称
SOX17 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human SOX17 aa 1-50 / 414.
种属反应性
Human, Mouse, Rat
验证应用
WB, FC
分子量
44 kDa
阳性对照
SKOV-3 cell lysate, HepG2 cell lysate, Hela cell lysate, mouse testis tissue lysate, rat testis tissue lysate, rat brain tissue lysate, Hela.
偶联
unconjugated
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
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1:500-1:2,000
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FC
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1:500-1:1,000
靶点
功能
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Acts as transcription regulator that binds target promoter DNA and bends the DNA. Binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays a key role in the regulation of embryonic development. Required for normal development of the definitive gut endoderm. Required for normal looping of the embryonic heart tube. Plays an important role in embryonic and postnatal vascular development, including development of arteries. Plays an important role in postnatal angiogenesis, where it is functionally redundant with SOX18. Required for the generation and maintenance of fetal hematopoietic stem cells, and for fetal hematopoiesis. Probable transcriptional activator in the premeiotic germ cells.
背景文献
1. Tan DS. et. al. SOX17 in cellular reprogramming and cancer. Semin Cancer Biol. 2020 Dec
2. Liu M. et. al. Sox17 is required for endothelial regeneration following inflammation-induced vascular injury. Nat Commun. 2019 May
亚细胞定位
Nucleus.
别名
FLJ22252 antibody
SOX17 antibody
SOX17_HUMAN antibody
SRY (sex determining region Y) box 17 antibody
SRY box 17 antibody
SRY related HMG box transcription factor SOX17 antibody
Transcription factor SOX-17 antibody
Transcription factor SOX17 antibody
VUR3 antibody
图片
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Western blot analysis of SOX17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500476, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SKOV-3 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Hela cell lysate
Lane 4: Mouse testis tissue lysate
Predicted band size: 44 kDa
Observed band size: 55 kDa -
Western blot analysis of SOX17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500476, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat testis tissue lysate
Lane 2: Rat brain tissue lysate
Predicted band size: 44 kDa
Observed band size: 55 kDa -
Flow cytometric analysis of Hela cells labeling SOX17.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500476, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"