RMB: 900 特惠 1500 1500
产品规格
概述
产品名称
DDIT3 Recombinant Rabbit Monoclonal Antibody [JM10-31]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human DDIT3 aa 135-169 / 169.
种属反应性
Human, Mouse, Rat
验证应用
WB, FC
分子量
Predicted band size: 19 kDa
阳性对照
SW480 cell lysate, HeLa cell lysate, LOVO cell lysate, PC-12 cell lysate, Hela.
偶联
unconjugated
克隆号
JM10-31
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
-
FC
-
1:50-1:100
靶点
功能
DNA damage-inducible transcript 3, also known as C/EBP homologous protein (CHOP), is a pro-apoptotic transcription factor that is encoded by the DDIT3 gene. It is a member of the CCAAT/enhancer-binding protein (C/EBP) family of DNA-binding transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis and has an important role in the cell's stress response. The regulation of CHOP expression plays an important role in metabolic diseases and in some cancers through its function in mediating apoptosis. The regulation of CHOP expression could be a potential approach to affecting cancer cells through the induction of apoptosis. In the intestinal epithelium, CHOP has been demonstrated to be downregulated under inflammatory conditions (in inflammatory bowel diseases and experimental models of colitis). In this context, CHOP seems to rather regulate the cell cycle than apoptotic processes. Mutations or fusions of CHOP (e.g. with FUS to form FUS-CHOP) can cause Myxoid liposarcoma.
背景文献
1. Sun XY et al. Valproate attenuates diabetic nephropathy through inhibition of endoplasmic reticulum stress-induced apoptosis. Mol Med Rep 13:661-8 (2016).
2. Greenwood M et al. Transcription Factor CREB3L1 Regulates Endoplasmic Reticulum Stress Response Genes in the Osmotically Challenged Rat Hypothalamus. PLoS One 10:e0124956 (2015).
序列相似性
Belongs to the bZIP family.
翻译后修饰
Ubiquitinated, leading to its degradation by the proteasome.; Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity.
亚细胞定位
Cytoplasm, Nucleus.
别名
C/EBP homologous protein antibody
C/EBP Homology Protein antibody
C/EBP zeta antibody
C/EBP-homologous protein 10 antibody
C/EBP-homologous protein antibody
CCAAT/enhancer binding protein homologous protein antibody
CEBPZ antibody
CHOP 10 antibody
CHOP antibody
CHOP-10 antibody
展开C/EBP homologous protein antibody
C/EBP Homology Protein antibody
C/EBP zeta antibody
C/EBP-homologous protein 10 antibody
C/EBP-homologous protein antibody
CCAAT/enhancer binding protein homologous protein antibody
CEBPZ antibody
CHOP 10 antibody
CHOP antibody
CHOP-10 antibody
CHOP10 antibody
DDIT 3 antibody
DDIT-3 antibody
Ddit3 antibody
DDIT3_HUMAN antibody
DNA Damage Inducible Transcript 3 antibody
DNA damage-inducible transcript 3 protein antibody
GADD 153 antibody
GADD153 antibody
Growth Arrest and DNA Damage Inducible Protein 153 antibody
Growth arrest and DNA damage inducible protein GADD153 antibody
Growth arrest and DNA damage-inducible protein GADD153 antibody
MGC4154 antibody
折叠图片
-
Western blot analysis of DDIT3 on different lysates with Rabbit anti-DDIT3 antibody (ET1703-05) at 1/5,000 dilution.
Lane 1: SW480 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Predicted band size: 19 kDa
Observed band size: 27 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-05) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of DDIT3 on different lysates with Rabbit anti-DDIT3 antibody (ET1703-05) at 1/1,000 dilution.
Lane 1: SW480-si NT cell lysate
Lane 2: SW480-si DDIT3 cell lysate
Lysates/proteins at 3 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 25 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
ET1703-05 was shown to specifically react with DDIT3 in SW480-si NT cells. Weakened band was observed when SW480-si DDIT3 sample was tested. SW480-si NT and SW480-si DDIT3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1703-05, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-05, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: LOVO cell lysate
Lane 2: PC-12 cell lysate
Predicted band size: 19 kDa
Observed band size: 25 kDa -
Flow cytometric analysis of DDIT3 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-05, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Characteristics of endoplasmic reticulum stress changes during the differentiation of adipose-derived stromal cells into neurons
期刊: Cytotechnology
DOI: 10.1007/s10616-025-00891-8
IF: 1.7
应用: IHC,WB
反应种属: Human
发表时间: 2026 Jan
-
Agarononaose from red algae delays cardiomyocyte aging by improving mitochondrial function via enhanced oxidative phosphorylation: A mitochondrial transplantation perspective
期刊: Journal Of Functional Foods
DOI: 10.1016/j.jff.2025.107131
IF: 4
应用: WB
反应种属: Mouse
发表时间: 2026 Jan
-
PF4/CXCR3 signaling contributes to the regulation of endothelial cells on smooth muscle cells in CKD vascular calcification
期刊: International Immunopharmacology
DOI: 10.1016/j.intimp.2026.116235
IF: 4.7
应用: WB
反应种属: Rat
发表时间: 2026 Jan
-
Ethanol-induced liver injury is alleviated by chrysin via increasing MRP2 transporter expression and enhancing the Nrf2-mediated adaptive response
期刊: Naunyn-Schmiedebergs Archives Of Pharmacology
DOI: 10.1007/s00210-025-04774-9
IF: 3.1
应用: WB
反应种属: Rat
发表时间: 2025 Oct
-
Sappanchalcone Suppresses NSCLC by Oxidative Stress-Driven DNA Damage and ER Stress Activation through PIEZO1 Modulation
期刊: iScience
DOI: 10.1016/j.isci.2025.114057
IF: 4.1
应用: WB
反应种属: Human
发表时间: 2025 Nov
-
Endoplasmic Reticulum-Targeted Photodynamic Therapy Combined with Autophagy Inhibition to Enhance Synergistic Immunotherapy for Triple-Negative Breast Cancer
期刊: Chemistry - A European Journal
DOI: 10.1002/chem.202500146
IF: 3.9
应用: WB,IF
反应种属: Mouse
发表时间: 2025 May
-
Vitamin E alleviates ammonia‑nitrogen and hypoxia co-exposure-induced intestinal damage in grass carp (Ctenopharyngodon idella) by modulating mitochondrial function, cellular autophagy and apoptosis
期刊: Aquaculture
DOI: 10.1016/j.aquaculture.2025.742868
IF: 3.9
应用: WB
反应种属:
发表时间: 2025 Jun
-
Perfluorohexane sulfonate (PFHxS) induces liver cytotoxicity by activating autophagy and endoplasmic reticulum stress-regulated mitochondrial pathway apoptosis
期刊: Food And Chemical Toxicology
DOI: 10.1016/j.fct.2025.115657
IF: 3.5
应用: WB
反应种属: Human
发表时间: 2025 Jul
-
Nootkatone inhibits the progression of glioblastoma by activating the ATF4-CHOP-CHAC1 pathway
期刊: Molecular Medicine
DOI:
IF: 6
应用: WB
反应种属: Mouse,Human
发表时间: 2025 Jan
-
Nootkatone inhibits the progression of glioblastoma by activating the ATF4-CHOP-CHAC1 pathway
期刊: MOLECULAR MEDICINE
DOI: 10.1186/s10020-025-01064-1
IF: 6.4
应用: WB
反应种属: Mouse,Human
发表时间: 2025 Jan
-
Tumor Microenvironment-Driven Structural Transformation of Vanadium-Based MXenzymes to Amplify Oxidative Stress for Multimodal Tumor Therapy
期刊: Advanced Science
DOI: 10.1002/advs.202408998
IF: 14.1
应用: WB
反应种属: Mouse
发表时间: 2025 Jan
-
Tumor Microenvironment-Driven Structural Transformation of Vanadium-Based MXenzymes to Amplify Oxidative Stress for Multimodal Tumor Therapy
期刊: Advanced Science
DOI:
IF: 14.3
应用: WB
反应种属: Mouse
发表时间: 2025 Jan
-
Single-nucleus RNA sequencing decodes abnormal cell-collagen communication in a sheep endometrial fibrosis model
期刊: International Journal Of Biological Macromolecules
DOI: 10.1016/j.ijbiomac.2025.140628
IF: 7.7
应用: WB
反应种属: Sheep
发表时间: 2025 Feb
-
Cyanidin-3-O-Glucoside Mitigates Hepatotoxicity Induced by 2-Amino-3-Methylimidazo[4,5-f]Quinoline via Endogenous and Exogenous Apoptotic Signaling Pathways: Evidence From In Vivo and In Silico Studies
期刊: Molecular Nutrition & Food Research
DOI: 10.1002/mnfr.70363
IF: 4.2
应用: WB
反应种属: Mouse
发表时间: 2025 Dec
-
Multi-Omics Analysis Reveals Distinct Lipid Remodelling and Mitochondrial Stress in SH-SY5Y Cells Modelling Parkinson’s Disease
期刊: Metabolites
DOI: 10.3390/metabo15120781
IF: 3.7
应用: WB
反应种属: Human
发表时间: 2025 Dec
-
GPX2 inhibition enhances antitumor efficacy of lenvatinib via promoting immunogenic cell death in hepatocellular carcinoma
期刊: Journal Of Translational Medicine
DOI: 10.1186/s12967-025-06468-5
IF: 6.1
应用: WB
反应种属: Human,Mouse
发表时间: 2025 Apr
-
Novel immunogenic cell death inducer combined with autophagy inhibitor to amplify photodynamic synergistic immunotherapy for triple-negative breast cancer
期刊: Research Square
DOI: 10.21203/rs.3.rs-4950334/v1
IF:
应用: WB
反应种属: Mouse
发表时间: 2024 Oct
-
Particulate matter induced cognitive impairments via endoplasmic reticulum stress-mediated damage to mitochondria-associated endoplasmic reticulum membranes in immature rats
期刊: Toxicology
DOI:
IF: 4.8
应用: WB
反应种属: Rat
发表时间: 2024 Oct
-
Endoplasmic reticulum stress is attenuated by glycolysis in lymphatic malformations
期刊: Pediatric Research
DOI:
IF: 3.6
应用: WB
反应种属: Human
发表时间: 2024 May
-
Discovery of novel peptide-dehydroepiandrosterone hybrids inducing endoplasmic reticulum stress with effective in vitro and in vivo anti-melanoma activities
期刊: European Journal Of Medicinal Chemistry
DOI:
IF: 6.7
应用: WB
反应种属: Mouse
发表时间: 2024 Mar
-
HDAC1 Promotes Mitochondrial Pathway Apoptosis and Inhibits the Endoplasmic Reticulum Stress Response in High Glucose-Treated Schwann Cells via Decreased U4 Spliceosomal RNA
期刊: Neurochemical Research
DOI:
IF: 3,7
应用: WB
反应种属: Rat
发表时间: 2024 Jul
-
In vivo therapy of osteosarcoma using anion transporters-based supramolecular drugs
期刊: Journal Of Nanobiotechnology
DOI:
IF: 10.2
应用: WB
反应种属: Mouse
发表时间: 2024 Jan
-
Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice
期刊: FEBS Journal
DOI:
IF: 5.4
应用: WB
反应种属: Mouse
发表时间: 2023 May
-
Excessive ER-phagy contributes to ochratoxin A-induced apoptosis
期刊: Food And Chemical Toxicology
DOI:
IF: 4.3
应用: WB
反应种属: Species independent,Human
发表时间: 2023 Jun
-
Porcine epidemic diarrhea virus activates PERK-ROS axis to benefit its replication in Vero E6 cells
期刊: Veterinary Research
DOI:
IF: 3.829
应用: WB
反应种属: African green monkey
发表时间: 2023 Feb
-
Tunicamycin induces depression-like behaviors in male rats, accompanied by initiated chaperon-mediated autophagy and decreased synaptic protein expression in the hippocampus
期刊: Neuroscience Letters
DOI:
IF:
应用: WB
反应种属: Rat
发表时间: 2023 Feb
-
GRAMD4 regulates PEDV-induced cell apoptosis inhibiting virus replication via the endoplasmic reticulum stress pathway
期刊: Veterinary Microbiology
DOI:
IF:
应用: WB
反应种属: monkey
发表时间: 2023 Apr
-
Porcine circovirus type 2 induces CHOP-ERO1α-ROS-mediated apoptosis in PK-15 cells
期刊: Veterinary Microbiology
DOI:
IF: 3.3
应用: WB
反应种属: Pig
发表时间: 2022 Oct
-
Corn peptides ameliorate nonalcoholic fatty liver disease by suppressing endoplasmic reticulum stress via the AMPKα/Sirt1 pathway in vivo and in vitro
期刊: Journal Of Functional Foods
DOI:
IF: 4.451
应用: WB
反应种属: Rat
发表时间: 2022 Jun
-
Nrf2 loss of function exacerbates endoplasmic reticulum stress-induced apoptosis in TBI mice
期刊: Neuroscience Letters
DOI:
IF: 2.5
应用: IHC-P,WB
反应种属: Mouse
发表时间: 2022 Jan
-
Site-1 protease controls osteoclastogenesis by mediating LC3 transcription. Cell death and differentiation, 28(6), 2001–2018.
期刊: Cell Death & Differentiation
DOI:
IF: 10.717
应用: WB,IF,IP
反应种属: Mouse
发表时间: 2021 Jun
-
Genetic inhibition of FABP4 attenuated endoplasmic reticulum stress and mitochondrial dysfunction in rhabdomyolysis-induced acute kidney injury
期刊: Life Sciences
DOI: 10.1016/j.lfs.2021.119023
IF: 5.037
应用: IHC-P
反应种属: Mouse
发表时间: 2021 Jan
-
Heat stress induced IPEC-J2 cells barrier dysfunction through endoplasmic reticulum stress mediated apoptosis by p-eif2α/CHOP pathway
期刊: Journal Of Cellular Physiology
DOI:
IF: 6.384
应用: WB
反应种属: Pig
发表时间: 2021 Feb
-
<html><html><html>Mettl14-Mediated m<sup>6</sup>A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis. Cellular and molecular gastroenterology and hepatology, S2352-345X(21)00072-2. Advance online publication.</html></html></html>
期刊: Cellular And Molecular Gastroenterology And Hepatology
DOI:
IF: 7.076
应用: WB
反应种属: Mouse
发表时间: 2021 Apr
-
Lipofectamine 2000/siRNA complexes cause endoplasmic reticulum unfolded protein response in human endothelial cells. Journal of cellular physiology, 234(11), 21166–21181.
期刊: Journal Of Cellular Physiology
DOI:
IF: 4.522
应用: WB,IF
反应种属: Human
发表时间: 2019 Nov
-
Intracellular XBP1-IL-24 axis dismantles cytotoxic unfolded protein response in the liver
期刊: bioRxiv
DOI:
IF: 9.685
应用: WB,IHC-P
反应种属: Mouse
发表时间: 2019 Jan
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