PMS2 Recombinant Rabbit Monoclonal Antibody [SY08-09]

☑ Relative expression (RE)

Immunocytochemistry analysis of HeLa (positive) and HCT 116 (negative) cells labeling PMS2 with Rabbit anti-PMS2 antibody (ET1605-1) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PMS2 antibody (ET1605-1) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

☑ Relative expression (RE)

Western blot analysis of PMS2 on different lysates with Rabbit anti-PMS2 antibody (ET1605-1) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: HCT 116 cell lysate (negative) (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)

Predicted band size: 96 kDa
Observed band size: 120 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-1) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

☑ Relative expression (RE)

Western blot analysis of PMS2 on different lysates with Rabbit anti-PMS2 antibody (ET1605-1) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lane 5: HCT 116 cell lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 96 kDa
Observed band size: 120 kDa

Exposure time: 37 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-1) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

☑ Knockdown (KD)

Western blot analysis of PMS2 on different lysates with Rabbit anti-PMS2 antibody (ET1605-1) at 1/5,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-PMS2 KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 96 kDa
Observed band size: 100 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-1) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PMS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1605-1, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PMS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1605-1, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

☑ Relative expression (RE)

Flow cytometric analysis of HeLa (positive, red) and HCT 116 (negative, green) cells labeling PMS2.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET1605-1, red) at 1/1,000 dilution and competitor's antibody (red) at 1/200 dilution, compared with Rabbit IgG Isotype Control (HeLa black, HCT 116 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃.