NMDAR2B Rabbit Polyclonal Antibody
Catalog# ER1913-90
NMDAR2B Rabbit Polyclonal Antibody
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WB
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ELISA
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IHC-P
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IF
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ICC
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Human
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Mouse
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Rat
概述
产品名称
NMDAR2B Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human NMDAR2B 451-550/1484
种属反应性
Human, Mouse, Rat
验证应用
WB, ELISA, IHC-P, IF, ICC
分子量
163 KDa
阳性对照
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-2000
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ELISA
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1:5000-10000
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IHC-P
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1:100-500
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IF
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1:100-500
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ICC
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1:100-500
发表文章中的应用
发表文章中的种属
靶点
功能
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death.
背景文献
暂无
亚细胞定位
Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
UNIPROT #
别名
AW490526 antibody
EIEE27 antibody
Glutamate [NMDA] receptor subunit epsilon 2 antibody
Glutamate [NMDA] receptor subunit epsilon-2 antibody
Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
Glutamate receptor ionotropic NMDA2B antibody
Glutamate receptor subunit epsilon 2 antibody
Glutamate receptor
ionotropic
展开图片
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Sample: A549 Cell Lysate at 40 ug
Primary: Anti- NMDAR2B (ER1913-90) at 1/300 dilution
Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 163 kD
Observed band size: 210 kD -
Tissue/cell: rat spinal cord; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated(ER1913-90) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining -
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated(ER1913-90) 1:300, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Scorpion venom heat-resistant synthesized peptide ameliorates epileptic seizures and imparts neuroprotection in rats mediated by NMDA receptors
Author: Sui Aoran,et al
PMID: 38830458
应用: IF,WB
反应种属: Rat
发表时间: 2024 Jun
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Citation
Alternative Products
NMDAR2B Recombinant Rabbit Monoclonal Antibody [PSH05-46]
Application: WB,IHC-P,IHC-Fr
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
NMDAR2B Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Mouse,Rat,Human
Conjugate: unconjugated
同靶点&同通路的产品
NMDAR2B Recombinant Rabbit Monoclonal Antibody [PSH05-46]
Application: WB,IHC-P,IHC-Fr
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated