CD105 Recombinant Rabbit Monoclonal Antibody [JE60-59]
Catalog# HA720072
CD105 Recombinant Rabbit Monoclonal Antibody [JE60-59]
-
WB
-
IHC-P
-
IF-Tissue
-
Human
概述
产品名称
CD105 Recombinant Rabbit Monoclonal Antibody [JE60-59]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human CD105 aa 1-250/658.
种属反应性
Human
验证应用
WB, IHC-P, IF-Tissue
分子量
Predicted band size: 71 kDa
阳性对照
HeLa cell lysate, HUVEC cell lysate, human placenta tissue, human spleen tissue, human kidney tissue.
偶联
unconjugated
克隆号
JE60-59
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IHC-P
-
1:200-1:500
-
IF-Tissue
-
1:200
靶点
功能
This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
背景文献
1. Wu HW. et. al. Anti-CD105 Antibody Eliminates Tumor Microenvironment Cells and Enhances Anti-GD2 Antibody Immunotherapy of Neuroblastoma with Activated Natural Killer Cells. Clin Cancer Res. 2019 Aug
2. Kauer J. et. al. CD105 (Endoglin) as negative prognostic factor in AML. Sci Rep. 2019 Dec
亚细胞定位
Cell membrane.
UNIPROT
别名
AI528660 antibody
AI662476 antibody
CD 105 antibody
CD105 antibody
CD105 antigen antibody
EGLN_HUMAN antibody
END antibody
Endoglin antibody
Eng antibody
FLJ41744 antibody
展开图片
-
☑ Cell treatment (CT)
Western blot analysis of CD105 on different lysates with Rabbit anti-CD105 antibody (HA720072) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa cell lysate treated with deglycosylation
Lane 3: HUVEC cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 71-100 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD105 antibody (HA720072) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720072) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD105 antibody (HA720072) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720072) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD105 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720072, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Application: IF-Tissue
Species: Human
Site: Spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/200
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
CD105 Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
CD105 Mouse Monoclonal Antibody [H4-A9]
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
