Sodium Potassium ATPase Recombinant Rabbit Monoclonal Antibody [ST0533]
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Catalog# ET1609-76
Sodium Potassium ATPase Recombinant Rabbit Monoclonal Antibody [ST0533]
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
Sodium Potassium ATPase Recombinant Rabbit Monoclonal Antibody [ST0533]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human ATP1A1 aa 39-83 / 1023.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IF-Tissue, IHC-P
分子量
Predicted band size: 113 kDa
阳性对照
A549 cell lysates, HeLa cell lysate, HT-29 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, L-929 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HeLa, MCF-7, human liver tissue, human kidney tissue, mouse liver tissue, mouse kidney tissue, rat kidney tissue.
偶联
unconjugated
克隆号
ST0533
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:100,000-1:200,000
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IF-Cell
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1:100-1:500
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IHC-P
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1:2,000-1:5,000
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IF-Tissue
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1:200
发表文章中的应用
| WB | 查看 1 篇文献如下 |
发表文章中的种属
| Mouse | 查看 1 篇文献如下 |
靶点
功能
The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na+/K+-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na+/K+-ATPase.
背景文献
1. Yang SH et al. The lamellae-free-type pseudobranch of the euryhaline milkfish (Chanos chanos) is a Na(+), K(+)-ATPase-abundant organ involved in hypoosmoregulation. Comp Biochem Physiol A Mol Integr Physiol 170:15-25 (2014).
2. R der PV et al. The role of SGLT1 and GLUT2 in intestinal glucose transport and sensing. PLoS One 9:e89977 (2014).
序列相似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
翻译后修饰
Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity).
亚细胞定位
Cell membrane, Melanosome.
UNIPROT
别名
ATPase Na+/K+ transporting alpha antibody
adenosinetriphosphatase antibody
AT1A1_HUMAN antibody
ATP1A1 antibody
ATP1A4 antibody
ATP1AL2 antibody
ATP1B antibody
ATP1B1 antibody
ATPase Na+/K+ transporting alpha 1 polypeptide antibody
ATPase Na+/K+ transporting alpha 4 polypeptide antibody
展开图片
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Western blot analysis of Sodium Potassium ATPase on different lysates with Rabbit anti-Sodium Potassium ATPase antibody (ET1609-76) at 1/100,000 dilution and competitor's antibody at 1/100,000 dilution.
Lane 1: HeLa cell lysate (no heat) (20 µg/Lane)
Lane 2: HT-29 cell lysate (no heat) (20 µg/Lane)
Lane 3: HepG2 cell lysate (no heat) (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (no heat) (20 µg/Lane)
Lane 5: L-929 cell lysate (no heat) (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (no heat) (20 µg/Lane)
Lane 7: Rat brain tissue lysate (no heat) (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 113 kDa
Observed band size: 100 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-76) at 1/100,000 dilution and competitor's antibody at 1/100,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling Sodium Potassium ATPase with Rabbit anti-Sodium Potassium ATPase antibody (ET1609-76) at 1/500 dilution and competitor's antibody at 1/500 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Sodium Potassium ATPase antibody (ET1609-76) at 1/500 dilution and competitor's antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Knockdown (KD)
Western blot analysis of Sodium Potassium ATPase on different lysates with Rabbit anti-Sodium Potassium ATPase antibody (ET1609-76) at 1/10,000 dilution.
Lane 1: Hela-si NT cell lysate
Lane 2: Hela-si Sodium Potassium ATPase cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 113 kDa
Observed band size: 100 kDa
Exposure time: 15 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1609-76, 1/10,000) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Sodium Potassium ATPase antibody (ET1609-76) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-76) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Sodium Potassium ATPase antibody (ET1609-76) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-76) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Sodium Potassium ATPase with Rabbit anti-Sodium Potassium ATPase antibody (ET1609-76) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1609-76, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.
Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded rat kidney tissue labeling Sodium Potassium ATPase with Rabbit anti-Sodium Potassium ATPase antibody (ET1609-76) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1609-76, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.
Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunocytochemistry analysis of NIH/3T3 cells labeling Sodium Potassium ATPase with Rabbit anti-Sodium Potassium ATPase antibody (ET1609-76) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Sodium Potassium ATPase antibody (ET1609-76) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of C6 cells labeling Sodium Potassium ATPase with Rabbit anti-Sodium Potassium ATPase antibody (ET1609-76) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Sodium Potassium ATPase antibody (ET1609-76) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of C6 cells labeling Sodium Potassium ATPase.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1609-76, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Cholesterol removal improves performance of a model biomimetic system to co-deliver a photothermal agent and a STING agonist for cancer immunotherapy
Author: Lin Li, Mengxing Zhang, Jing Li, Tiantian Liu, Qixue Bao, Xi Li, Jiaying Long, Leyao Fu, Zhirong Zhang, Shiqi Huang, Zhenmi Liu, Ling Zhang
PMID: 37607938
应用: WB
反应种属: Mouse
发表时间: 2023 Aug
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Citation
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Minute Cellular Nodules as Early Lesions in Rats with Silica Exposure via Inhalation
Author: Li, Y., Jin, F., Li, T., Yang, X., Cai, W., Li, S., Gao, X., Mao, N., Liu, H., Xu, H., & Yang, F.
PMID: 35737303
应用:
反应种属:
发表时间: 2022 Jun
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Citation
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