Hippo Signaling Antibody Kit
Catalog# HAK21009
Hippo Signaling Antibody Kit
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WB
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Human
General Overview
Kit Components
| Product Includes | Specification | Application | Reactivity | Mw |
|---|---|---|---|---|
| MOB1A[ER61553] | 20µl | WB | Human,Mouse,Rat | 23 kDa |
| YAP1[ET1608-30] | 20µl | WB,IF-Cell,IF-Tissue,IHC-P,IP,FC | Human,Mouse,Rat | Predicted band size: 54 kDa |
| LATS1 / WARTS[HA722808] | 20µl | WB | Human,Mouse,Monkey | Predicted band size: 127 kDa |
| MST2[ET1610-8] | 20µl | WB,IHC-P,IP | Human,Mouse,Rat | Predicted band size: 56 kDa |
| MST1[HA500031] | 20µl | WB,IHC-P | Human,Mouse | Predicted band size: 57 kDa. |
| Phospho-YAP1 (S127)[ET1611-69] | 20µl | WB,IHC-P,IF-Tissue | Human,Mouse,Rat | Predicted band size: 54 kDa |
| TAZ[HA500300] | 20µl | WB,IHC-P,IF-Cell | Human,Mouse | 44 kDa |
| SAV1[HA722130] | 20µl | WB,IHC-P,FC | Human,Mouse,Rat | Predicted band size: 45 kDa |
| Goat Anti-Rabbit IgG (H+L)[HA1001] | 100µl | WB,ELISA,IHC-P | Rabbit |
Product Description
The Hippo Signaling Antibody Sampler Kit provides an economical means of detecting target proteins of the Hippo signaling pathway. The kit contains enough primary antibody to perform two western blots per primary.
Product Features
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Background
Hippo signaling is an evolutionarily conserved pathway that controls cell proliferation, apoptosis, and organ size in response to changing cell density levels. At relative low cell density, transcription co-activators YAP and TAZ bind transcription factors to induce expression of genes that favor cell growth and proliferation. As cell density increases, interaction between membrane-bound upstream hippo pathway regulators trigger activation of cytoplasmic kinases Mst1/2 and LATS1/2. Activated Mst kinase (the eponymous Hippo in Drosophila) associates with the adaptor Sav1 and phosphorylates MOB1 to activate LATS kinase, which phosphorylates YAP and TAZ to suppress cell proliferation.
Data Links
Background References
1. McNeill, H. and Woodgett, J.R. (2010) Nat Rev Mol Cell Biol 11, 404-13.
2. Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92.
3. Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
Images
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Western blot analysis of Phospho-YAP1 (S127) on different lysates with Rabbit anti-Phospho-YAP1 (S127) antibody (ET1611-69) at 1/5,000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes then treated with λpp for 1 hour whole cell lysate
Lane 4: NIH/3T3 whole cell lysate
Lane 5: NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes then treated with λpp for 1 hour whole cell lysate
Lysates/proteins at 10 µg/Lane.
Lane 7: C6 whole cell lysate
Lane 8: C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 9: C6 treated with 100nM Calyculin A for 30 minutes then treated with λpp for 1 hour whole cell lysate
Predicted band size: 54 kDa
Observed band size: 70 kDa
Exposure time: 1 minute 6 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-69) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of SAV1 on different lysates with Rabbit anti-SAV1 antibody (HA722130) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HCT 116 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Lane 5: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 6: SW480 cell lysate (20 µg/Lane)
Lane 7: BxPC-3 cell lysate (20 µg/Lane)
Lane 8: 786-0 cell lysate (negative) (20 µg/Lane)
Lane 9: A549 cell lysate (20 µg/Lane)
Lane 10: NIH/3T3 cell lysate (20 µg/Lane)
Lane 11: PC-12 cell lysate (20 µg/Lane)
Lane 12: Mouse testis tissue lysate (40 µg/Lane)
Lane 13: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 3 minute4; ECL: Lane 1-13 (left): K1801; Lane 1-13 (right): K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722130) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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