Exosomal Marker Antibody Kit
Catalog# HAK21005
Exosomal Marker Antibody Kit
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WB
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Human
General Overview
Kit Components
| Product Includes | Specification | Application | Reactivity | Mw |
|---|---|---|---|---|
| ALIX[ET1705-74] | 20µl | WB,IHC-P,FC,IF-Cell | Human,Mouse,Rat | Predicted band size: 96 kDa |
| Annexin V[ET1702-62] | 20µl | WB,FC | Human,Mouse,Rat | Predicted band size: 36 kDa |
| ICAM1[ET1609-46] | 20µl | WB,IF-Tissue,IHC-P | Human | Predicted band size: 58 kDa |
| CD9[ET1601-9] | 20µl | WB,IF-Cell,IHC-P,IP,FC | Human,Mouse,Rat | Predicted band size: 25 kDa |
| GM130[HA721282] | 20µl | WB,IHC-P,IF-Cell | Human,Mouse | Predicted band size: 113 kDa |
| EpCAM[ET1608-26] | 20µl | WB,IHC-P | Human,Mouse,Rat | Predicted band size: 35 kDa |
| Hsp70[ET1601-11] | 20µl | WB,IF-Cell,IF-Tissue,IHC-P,FC | Human,Mouse,Rat | Predicted band size: 70 kDa |
| Flotillin 1[ET7107-82] | 20µl | WB,IHC-P | Human,Mouse,Rat | Predicted band size: 47 kDa |
| Alpaca anti-Rabbit IgG Fc, Recombinant VHH[HA1031] | 100µl | IP,ELISA,IHC-P,WB | Rabbit |
Product Description
The Exosomal Marker Antibody Sampler Kit provides an economical method to evaluate the presence of exosome markers. The kit contains enough primary antibodies to perform two Western blotting experiments on each target.And also includes secondary reagent for detection of these antibodies.
Product Features
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Background
Exosomes are small membrane-bound vesicles that in recent years have emerged as important molecules for inter-cellular communication.The exosomal markers contained in this kit are Alix, Annexin V, ICAM-1, CD9, GM130, EpCAM, flotillin, and HSP70.</br> Alix regulates many cellular processes . Annexin V is a ~30 kDa protein that binds to phospho-lipids in a calcium-dependent manner. CD54 or ICAM-1 is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules.The CD9 antigen belongs to the tetraspanin family of cell surface glycoproteins. GM130 is required for membrane fusion events. EpCAM/CD326 is a transmembrane glycoprotein that mediates calcium-independent, Flotillins belong to a famiy of lipid raft-associated integral membrane proteins. HSP70 is a molecular chaperone expressed constituitively under normal conditions to maintain protein homeostatis and is induced upon environmental stress .
Data Links
Background References
1. Raposo, G. and Stoorvogel, W. (2013) J Cell Biol 200, 373-83.
2. Katoh, K. et al. (2003) J Biol Chem 278, 39104-13.
3. Sadoul, R. (2006) Biol Cell 98, 69-77.
4. Huber, R. et al. (1990) EMBO J 9, 3867-74.
5. Cardó-Vila, M. et al. (2003) Mol Cell 11, 1151-62.
6. Hopkins, A.M. et al. (2004) Adv Drug Deliv Rev 56, 763-78.
7. Hemler, M.E. (2005) Nat Rev Mol Cell Biol 6, 801-11.
8. Théry, C. et al. (1999) J Cell Biol 147, 599-610.
9. Puthenveedu, M.A. et al. (2006) Nat Cell Biol 8, 238-48.
Images
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Western blot analysis of ALIX on different lysates with Rabbit anti-ALIX antibody (ET1705-74) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: K-562 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: PC-3M cell lysate
Lane 5: MCF7 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 96 kDa
Observed band size: 100 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-74) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (ET1601-9) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: HCT 116 cell lysate (12 µg/Lane)
Lane 5: HepG2 cell lysate (20 µg/Lane)
Lane 6: Jurkat cell lysate (20 µg/Lane)
Lane 7: Raji cell lysate (20 µg/Lane)
Lane 8: Rat lung tissue lysate (20 µg/Lane)
Predicted band size: 25 kDa
Observed band size: 23 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-9) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Hsp70 on different lysates with Rabbit anti-Hsp70 antibody (ET1601-11) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Mouse testis tissue lysate
Lane 7: Rat testis tissue lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: Lane 1-4: 43 seconds; Lane 5-7: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-11) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Related Products
ALIX Recombinant Rabbit Monoclonal Antibody [JM85-31] - BSA and Azide free
Application: WB,IHC-P,FC,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
