Phospho-HER2 / ErbB2 (Y1248) Recombinant Rabbit Monoclonal Antibody [PSH04-03]
Catalog# HA722084
Phospho-HER2 / ErbB2 (Y1248) Recombinant Rabbit Monoclonal Antibody [PSH04-03]
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WB
-
IF-Cell
-
Human
概述
产品名称
Phospho-HER2 / ErbB2 (Y1248) Recombinant Rabbit Monoclonal Antibody [PSH04-03]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Tyr1248 of Human HER2.
种属反应性
Human
验证应用
WB, IF-Cell
分子量
Predicted band size: 138 kDa
阳性对照
SK-Br-3 cell lysate, SK-Br-3 starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate, HeLa starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate, HeLa cells starved for 4 hours add 200ng/mL EGF for 15 minutes.
偶联
unconjugated
克隆号
PSH04-03
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IF-Cell
-
1:100
靶点
功能
HER-2 (also called HER-2/neu, c-erbB2, ERBB2 or neu) is a transmembrane receptor tyrosine kinase. HER-2 is a proto-oncogene, i.e. its activation causes malignant transformation and increases the malignant potential (cell proliferation, invasiveness etc.) of the cells. Amplification of HER-2 gene invariably leads to over-expression of its protein product. The magnitude of over- expression is usually 10-100 folds or even more. Over-expressed HER-2 protein disturbs the HER-receptor family signalling networks, i.e. signalling mediated via EGFR receptor, HER-3 and HER-4. In tumours, HER-2 is over-expressed in 15-25% of primary breast cancers. Metastases usually have the same amplification status as the primary tumours. HER-2 amplification and over-expression are typical features of hormone receptor negative, rapidly growing histologic grade 2-3 tumours. Of the histologic types, Paget’s disease is almost invariably HER-2 positive, whereas only a small minority of lobular and tubular carcinomas shows HER-2 amplification. HER-2 amplification and over-expression can also be found in intestinal type gastric and gastroesophageal carcinomas, ovarian carcinomas, high grade endometrial carcinomas and some salivary duct tumours. Low-level copy number increases have been found also in rare cases of lung tumours. Because of its central importance in breast cancer therapy selection, standardization of HER-2 IHC assays and slide interpretation are of outmost clinical and economical importance.
背景文献
1. Fernandez AI et al. Examination of Low ERBB2 Protein Expression in Breast Cancer Tissue. JAMA Oncol. 2022 Apr
2. Augustin JE et al. Targeting the complexity of ERBB2 biology in gastroesophageal carcinoma. Ann Oncol. 2022 Nov
亚细胞定位
Cell membrane, Cell projection, ruffle membrane; Early endosome, Cytoplasm, perinuclear region, Nucleus.
UNIPROT #
别名
Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
C erb B2/neu protein antibody
CD340 antibody
CD340 antigen antibody
Cerb B2/neu protein antibody
CerbB2 antibody
Erb b2 receptor tyrosine kinase 2 antibody
ErbB-2 proto-oncogene antibody
ERBB2 antibody
ERBB2_HUMAN antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of Phospho-HER2 / ErbB2 (Y1248) on different lysates with Rabbit anti-Phospho-HER2 / ErbB2 (Y1248) antibody (HA722084) at 1/1,000 dilution.
Lane 1: SK-Br-3 cell lysate
Lane 2: SK-Br-3 starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate
Lane 3: HeLa cell lysate
Lane 4: HeLa starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate
Lane 5: HeLa starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 138 kDa
Observed band size: 180 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722084) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells starved for 4 hours add 200ng/mL EGF for 15 minutes labeling Phospho-HER2 / ErbB2 (Y1248) with Rabbit anti-Phospho-HER2 / ErbB2 (Y1248) antibody (HA722084) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-HER2 / ErbB2 (Y1248) antibody (HA722084) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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