iFluor™ 488 Conjugated NeuN Recombinant Rabbit Monoclonal Antibody [SR45-07]
Catalog# HA720168F
iFluor™ 488 Conjugated NeuN Recombinant Rabbit Monoclonal Antibody [SR45-07]
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IF-Tissue
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Human
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Mouse
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Rat
概述
产品名称
iFluor™ 488 Conjugated NeuN Recombinant Rabbit Monoclonal Antibody [SR45-07]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human NeuN aa 20-60.
种属反应性
Human, Mouse, Rat
验证应用
IF-Tissue
分子量
Predicted band size: 34 kDa
阳性对照
Mouse brain tissue.
偶联
iFluor™ 488
克隆号
SR45-07
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IF-Tissue
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1:50
靶点
功能
Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.
背景文献
1. Patel TP et al. Single-neuron NMDA receptor phenotype influences neuronal rewiring and reintegration following traumatic injury. J Neurosci 34:4200-13 (2014).
2. Kaur P et al. Expression profiling of RNA transcripts during neuronal maturation and ischemic injury. PLoS One 9:e103525 (2014).
亚细胞定位
Cytoplasm, Nucleus
别名
FLJ56884 antibody
FLJ58356 antibody
Fox-1 homolog C antibody
fox1 homolog C antibody
Fox3 antibody
FOX3NeuN antibody
hexaribonucleotide binding protein 3 antibody
HRNBP3 antibody
NEUN antibody
neuronal nuclei antibody
展开图片
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Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NeuN (HA720168F).
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (HA720168F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点&同通路的产品
NeuN Recombinant Mouse Monoclonal Antibody [PD01-45]
Application: WB,IF-Tissue,IHC-P,IHC-Fr
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
iFluor™ 594 Conjugated NeuN Recombinant Rabbit Monoclonal Antibody [SR45-07]
Application: IF-Tissue,IHC-Fr
Reactivity: Human,Mouse,Rat
Conjugate: iFluor™ 594
