NCAM1 Rabbit Polyclonal Antibody
Catalog# R1204-1
NCAM1 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Human
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Mouse
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Rat
概述
产品名称
NCAM1 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide corresponding to Mouse NCAM1 aa 101-150 / 1,115 mouse.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell
分子量
Predicted band size: 95 kDa
阳性对照
SH-SY5Y cell lysate, NCCIT cell lysate, F9 cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, SH-SY5Y, N2A.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:1,000
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IF-Cell
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1:50
靶点
功能
Neural Cell Adhesion Molecule (NCAM, also the cluster of differentiation CD56) is a homophilic binding glycoprotein expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory. NCAM is a glycoprotein of Immunoglobulin (Ig) superfamily. The three main isoforms of NCAM vary only in their cytoplasmic domain: NCAM-120kDa (GPI anchored), NCAM-140kDa (short cytoplasmic domain) and NCAM-180kDa (long cytoplasmic domain).
背景文献
1. "Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56)."Lanier L.L., Chang C., Azuma M., Ruitenberg J.J., Hemperly J.J., Phillips J.H.J. Immunol. 146:4421-4426(1991)
2. "Phosphoproteomic analysis of synaptosomes from human cerebral cortex."DeGiorgis J.A., Jaffe H., Moreira J.E., Carlotti C.G. Jr., Leite J.P., Pant H.C., Dosemeci A.J. Proteome Res. 4:306-315(2005)
亚细胞定位
Cell membrane, secreted
别名
antigen recognized by monoclonal antibody
5.1H11 antibody
CD56 antibody
Cell adhesion molecule, neural, 1 antibody
MSK39 antibody
N-CAM 140 antibody
N-CAM-1 antibody
NCAM antibody
NCAM-1 antibody
NCAM1 antibody
展开图片
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Western blot analysis of NCAM1 on different lysates with Rabbit anti-NCAM1 antibody (R1204-1) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: NCCIT cell lysate (20 µg/Lane)
Lane 3: F9 cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 95 kDa
Observed band size: 120-180 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1204-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of SH-SY5Y cells labeling NCAM1 with Rabbit anti-NCAM1 antibody (R1204-1) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NCAM1 antibody (R1204-1) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunocytochemistry analysis of N2A cells labeling NCAM1 with Rabbit anti-NCAM1 antibody (R1204-1) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NCAM1 antibody (R1204-1) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点&同通路的产品
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Conjugate: unconjugated
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Conjugate: unconjugated
