iFluor™ 488 Conjugated Lamin B1 Recombinant Rabbit Monoclonal Antibody [SI17-07]
Catalog# HA720171F
iFluor™ 488 Conjugated Lamin B1 Recombinant Rabbit Monoclonal Antibody [SI17-07]
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IF-Tissue
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Human
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Mouse
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Rat
概述
产品名称
iFluor™ 488 Conjugated Lamin B1 Recombinant Rabbit Monoclonal Antibody [SI17-07]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Lamin B1 aa 511-560 / 586.
种属反应性
Human, Mouse, Rat
验证应用
IF-Tissue
分子量
Predicted band size: 66 kDa
阳性对照
Human colon carcinoma tissue, mouse colon tissue, rat colon tissue.
偶联
iFluor™ 488
克隆号
SI17-07
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
IF-Tissue
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1:200
靶点
功能
A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/ICE, functions as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology as components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.
背景文献
1. Cruz OH et al. Multinucleation and Polykaryon Formation is Promoted by the EhPC4 Transcription Factor in Entamoeba histolytica. Sci Rep 6:19611 (2016).
2. Lund K et al. Slug-dependent upregulation of L1CAM is responsible for the increased invasion potential of pancreatic cancer cells following long-term 5-FU treatment. PLoS One 10:e0123684 (2015).
序列相似性
Belongs to the intermediate filament family.
翻译后修饰
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
亚细胞定位
Nucleus inner membrane.
别名
ADLD antibody
lamin B1 antibody
Lamin-B1 antibody
LMN antibody
LMN2 antibody
LMNB antibody
Lmnb1 antibody
LMNB1_HUMAN antibody
MGC111419 antibody
OTTHUMP00000159218 antibody
图片
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Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Lamin B1 with Rabbit anti-Lamin B1 antibody (HA720171F) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720171F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Lamin B1 with Rabbit anti-Lamin B1 antibody (HA720171F) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720171F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded rat colon tissue labeling Lamin B1 with Rabbit anti-Lamin B1 antibody (HA720171F) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720171F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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