Glucagon Recombinant Rabbit Monoclonal Antibody [JF33-10]
Catalog# ET1702-59
Glucagon Recombinant Rabbit Monoclonal Antibody [JF33-10]
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
Glucagon Recombinant Rabbit Monoclonal Antibody [JF33-10]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human GLP1 aa 65-150.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IF-Tissue, IHC-P
分子量
Predicted band size: 21 kDa
阳性对照
PC-12, SW480, human pancreas tissue.
偶联
unconjugated
克隆号
JF33-10
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1
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500-1:1,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:100
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IHC-P
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1:50-1:200
靶点
功能
Glucagon is a pancreatic hormone that functions as an antagonist to insulin, stimulating the conversion of glycogen to glucose and increasing blood sugar levels. Glucagon-like peptide-1 (GLP-1), Glucagon-like peptide-2 (GLP-2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are members of the Glucagon family of hormones. GLP-1 functions as a transmitter in the central nervous system, inhibiting feeding and drinking behavior, whereas GLP-2 is a stimulator of intestinal epithelial growth. VIP causes vasodilation resulting in the lowering of blood pressure. PACAP is abundant in the hypothalamus and has been shown to increase the synthesis of several hormones, including growth hormone.
背景文献
1. Takada Y et al. Brg1 plays an essential role in development and homeostasis of the duodenum through regulation of Notch signaling. Development 143:3532-3539 (2016).
2. Jiang S et al. AMPK-dependent regulation of GLP1 expression in L-like cells. J Mol Endocrinol 57:151-60 (2016).
序列相似性
Belongs to the glucagon family.
组织特异性
Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain.
翻译后修饰
Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
亚细胞定位
Secreted.
别名
GCG antibody
glicentin-related polypeptide antibody
GLP-1 antibody
GLP-1(7-36) antibody
GLP-1(7-37) antibody
GLP-2 antibody
GLP1 antibody
GLP1, included antibody
GLP2 antibody
GLP2, included antibody
展开图片
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Immunofluorescence analysis of paraffin-embedded mouse pancreas tissue labeling Glucagon with Rabbit anti-Glucagon antibody (ET1702-59) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-59, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
ICC staining GLP1 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining GLP1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GLP1 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点&同通路的产品
Glucagon Recombinant Rabbit Monoclonal Antibody [JF0960]
Application: WB,IHC-P,mIHC,IHC-Fr,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
