Fatty Acid Synthase Rabbit Polyclonal Antibody
Catalog# R1706-8
Fatty Acid Synthase Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
Fatty Acid Synthase Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within human Fatty Acid Synthase aa 801-1050.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IHC-P
分子量
273 kDa
阳性对照
A549, MCF-7, Hela, human prostate tissue, human breast cancer tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
发表文章中的应用
发表文章中的种属
| Mouse | See 2 publications below |
靶点
功能
Fatty acid biosynthesis is mediated by seven catalytic enzymes and an acyl carrier protein (ACP), to which various acyl intermediates are covalently attached. Fatty Acid Synthase (FAS) is the anabolic enzyme that contains the seven unique catalytic sites and mediates the conversion of acetyl-CoA and malonyl-CoA, in the presence of the cofactor NADPH, into long-chain saturated fatty acids, such as palmitate. Human Fatty Acid Synthase cDNA encodes a 2,504 amino acid protein. Catalytically active Fatty Acid Synthase is a homodimer. Human Fatty Acid Synthase mRNA is variably expressed with abundant levels present in brain, lung and liver. Fatty acid synthetic metabolism is abnormally elevated in tumor cells and may support cell growth or survival of malignant cancers.
背景文献
1. Sun X et al. Insulin Dissociates the Effects of Liver X Receptor on Lipogenesis, Endoplasmic Reticulum Stress, and Inflammation. J Biol Chem 291:1115-22 (2016).
2. Rolyan H et al. Defects of Lipid Synthesis Are Linked to the Age-Dependent Demyelination Caused by Lamin B1 Overexpression. J Neurosci 35:12002-17 (2015).
组织特异性
Ubiquitous. Prominent expression in brain, lung, and liver.
亚细胞定位
Cytoplasm. Melanosome.
别名
[Acyl-carrier-protein] S acetyltransferase antibody
[Acyl-carrier-protein] S malonyltransferase antibody
3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
3-oxoacyl-[acyl-carrier-protein] reductase antibody
3-oxoacyl-[acyl-carrier-protein] synthase antibody
Enoyl-[acyl-carrier-protein] reductase antibody
FAS antibody
FAS_HUMAN antibody
FASN antibody
Fatty acid synthase antibody
展开图片
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☑ Knockdown (KD)
All lanes: Western blot analysis of Fatty Acid Synthase with anti-Fatty Acid Synthase antibody (R1706-8) at 1:1,000 dilution.
Lane 1: Wild-type Hela whole cell lysate.
Lane 2: FASN knockdown Hela whole cell lysate.
R1706-8 was shown to specifically react with FASN in wild-type Hela cells. Weakened band was observed when FASN knockdown samples were tested. Wild-type and FASN knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-FASN antibody (R1706-8, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China). -
Western blot analysis of Fatty Acid Synthase on A549 cell lysate using anti-Fatty Acid Synthase antibody at 1/1,000 dilution.
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ICC staining Fatty Acid Synthase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining Fatty Acid Synthase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Combined Use of Bicyclol and Berberine Alleviates Mouse Nonalcoholic Fatty Liver Disease
Author: Li, H., Liu, N. N., Li, J. R., Dong, B., Wang, M. X., Tan, J. L., Wang, X. K., Jiang, J., Lei, L., Li, H. Y., Sun, H., Jiang, J. D., & Peng, Z. G.
PMID: 35250593
应用: WB
反应种属: Mouse
发表时间: 2022 Feb
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Citation
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Anlotinib suppresses lung adenocarcinoma growth via inhibiting FASN-mediated lipid metabolism
Author: Shen, J., Huang, J., Huang, Y., Chen, Y., Li, J., Luo, P., Zhang, Q., Qiu, Y., Wang, L., Jiang, H., Ma, S., & Chen, X.
PMID: 36660682
应用: WB,IHC-P
反应种属: Mouse
发表时间: 2022 Dec
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Citation
Alternative Products
Fatty Acid Synthase Recombinant Rabbit Monoclonal Antibody [JJ0939]
Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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