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E-cadherin Rabbit Polyclonal Antibody

RMB: 1500

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Catalog# ER63312

E-cadherin Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

E-cadherin Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

The antiserum was produced against synthesized peptide derived from human Cadherin. AA range:833-882

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P, IF

分子量

97 kDa

阳性对照

Brain,Epidermal carcinoma,Hippocampus,Liver,Pancreas,Placenta,Tongue,

偶联

unconjugated

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:500-1:2000

  • IHC-P

  • 1:100-1:300

  • IF

  • 1:100-1:500

发表文章中的应用

WB See 1 publications below
IHC See 1 publications below

发表文章中的种属

Mouse See 2 publications below

靶点

功能

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16.

背景文献

暂无

UNIPROT #

SwissProt: P12830 Human

SwissProt: P19022 Human

SwissProt: P22223 Human

SwissProt: P55283 Human

别名

Arc 1 antibody

CADH1_HUMAN antibody

Cadherin 1 antibody

cadherin 1 type 1 E-cadherin antibody

Cadherin-1 antibody

Cadherin1 antibody

CAM 120/80 antibody

CD 324 antibody

CD324 antibody

CD324 antigen antibody

展开

图片

  • Immunofluorescence analysis of rat-lung tissue. 1,E-cadherin Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

    Immunofluorescence analysis of rat-lung tissue. 1,E-cadherin Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

  • Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,E-cadherin Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

    Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,E-cadherin Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

  • Western Blot analysis of 293 cells using E-cadherin Polyclonal Antibody diluted at 1：2000

    Western Blot analysis of 293 cells using E-cadherin Polyclonal Antibody diluted at 1：2000

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IHC

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