CD4 Rabbit Polyclonal Antibody
Catalog# 0108-6
CD4 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
概述
产品名称
CD4 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within mouse CD4 aa 195-244 / 457.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, FC
分子量
Predicted band size: 51 kDa
阳性对照
Mouse brain tissue, LOVO, Raji.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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FC
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1:50-1:100
靶点
功能
The CD4 gene encodes the T4 antigen, a glycoprotein of approximately 55 kDa, expressed on the surface of helper T lymphocytes.CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in major histocompatibility complex class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as interleukin-15 receptors and bind to the HIV receptors, binding directly to the HIV envelope protein GP120.
背景文献
暂无
组织特异性
Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells.
翻译后修饰
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.; Phosphorylated by PKC; phosphorylation at Ser-433 plays an important role for CD4 internalization.
亚细胞定位
Cell membrane.
别名
CD 4 antibody
CD4 (L3T4) antibody
CD4 antibody
CD4 antigen (p55) antibody
CD4 antigen antibody
CD4 molecule antibody
CD4 receptor antibody
CD4+ Lymphocyte deficiency, included antibody
CD4_HUMAN antibody
CD4mut antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of CD4 on different lysates with Rabbit anti-CD4 antibody (0108-6) at 1/1,000 dilution.
Lane 1: THP-1-si NT cell lysate
Lane 2: THP-1-si CD4 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0108-6) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CD4 on mouse brain lysate using anti-CD4 antibody at 1/500 dilution.
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ICC staining CD4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Flow cytometric analysis of Raji cells with CD4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
CD4 Recombinant Rabbit Monoclonal Antibody [ST0488]
Application: WB,IF-Cell,IF-Tissue,IHC-P,FC,mIHC
Reactivity: Human
Conjugate: unconjugated
同靶点&同通路的产品
CD4 Recombinant Rabbit Monoclonal Antibody [ST0488]
Application: WB,IF-Cell,IF-Tissue,IHC-P,FC,mIHC
Reactivity: Human
Conjugate: unconjugated
CD4 Recombinant Rabbit Monoclonal Antibody [JE56-36]
Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
CD4 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
CD4
Application:
Reactivity:
Conjugate:
CD4 Rabbit Polyclonal Antibody
Application: WB,IF-Cell
Reactivity: Human
Conjugate: unconjugated
