CD38 Recombinant Rabbit Monoclonal Antibody [SN07-20]
Catalog# ET1611-66
CD38 Recombinant Rabbit Monoclonal Antibody [SN07-20]
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WB
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IHC-P
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FC
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IF-Tissue
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Human
概述
产品名称
CD38 Recombinant Rabbit Monoclonal Antibody [SN07-20]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human CD38 aa 251-300 / 300.
种属反应性
Human
验证应用
WB, IHC-P, FC, IF-Tissue
分子量
Predicted band size: 34 kDa
阳性对照
THP-1 cell lysates, Raji cell lysates, THP-1, human prostate tissue, human tonsil tissue, human peripheral blood lymphocytes, human appendix tissue.
偶联
unconjugated
克隆号
SN07-20
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
-
1:1,000
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IHC-P
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1:8,000-1:50,000
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FC
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1:1,000
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IF-Tissue
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1:1,000
靶点
功能
CD38 is a type II integral membrane glycoprotein which is present on early B and T cell lineages and activated B and T cells but is absent from most mature resting peripheral lymphocytes. CD38 is also found on thymocytes, pre-B cells, germinal center B cells, mitogen-activated T cells, monocytes and Ig-secreting plasma cells. CD38 acts as a NAD glycohydrolase in T lymphocytes. On hematopoietic cells CD38 induces activation, proliferation, and differentiation of mature T and B cells and mediates apoptosis of myeloid and lymphoid progenitor cells. In addition to acting as a signaling receptor, CD38 is also an enzyme capable of producing several calcium-mobilizing metabolites, including cyclic adenosine diphosphate ribose (cADPR). CD38 also plays a role in maintaining survival of an invariant NK T (iNKT) cell subset that preferentially contributes to the maintenance of immunological tolerance.
背景文献
1. Chini CC et al. Targeting of NAD metabolism in pancreatic cancer cells: potential novel therapy for pancreatic tumors. Clin Cancer Res 20:120-30 (2014).
2. Kobayashi M et al. Clinicopathological study of primary biliary cirrhosis with interface hepatitis compared to autoimmune hepatitis. World J Gastroenterol 20:3597-608 (2014).
序列相似性
Belongs to the ADP-ribosyl cyclase family.
组织特异性
Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.
亚细胞定位
Membrane.
UNIPROT #
别名
Acute lymphoblastic leukemia cells antigen CD38 antibody
ADP ribosyl cyclase 1 antibody
ADP ribosyl cyclase antibody
ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
ADP-ribosyl cyclase 1 antibody
ADPRC 1 antibody
ADPRC1 antibody
cADPr hydrolase 1 antibody
CD 38 antibody
CD38 antibody
展开图片
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Western blot analysis of CD38 on THP-1 cell lysates with Rabbit anti-CD38 antibody (ET1611-66) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 45 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-66) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CD38 on Raji cell lysates with Rabbit anti-CD38 antibody (ET1611-66) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 45 kDa
Exposure time: 30 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-66) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of THP-1 cells labeling CD38.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (ET1611-66, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-CD38 antibody (ET1611-66) at 1/8,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-66) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD38 antibody (ET1611-66) at 1/50,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-66) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of human peripheral blood lymphocytes labeling CD38.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (ET1611-66, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling CD38 with Rabbit anti-CD38 antibody (ET1611-66) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1611-66, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded human appendix tissue labeling CD38 with Rabbit anti-CD38 antibody (ET1611-66) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1611-66, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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