CD23 Rabbit Polyclonal Antibody
Catalog# HA500386
CD23 Rabbit Polyclonal Antibody
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IHC-P
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FC
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Human
概述
产品名称
CD23 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human CD23 aa 250-321 / 321 (Extracellular).
种属反应性
Human
验证应用
IHC-P, FC
分子量
Predicted band size: 36 kDa
阳性对照
Human tonsil tissue, Raji.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
靶点
功能
The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
背景文献
1. Sutton BJ. et. al. Structure and dynamics of IgE-receptor interactions: FcεRI and CD23/FcεRII. Immunol Rev. 2015 Nov;268(1):222-35.
2. Selb R. et. al. Critical and direct involvement of the CD23 stalk region in IgE binding. J Allergy Clin Immunol. 2017 Jan;139(1).
亚细胞定位
Secreted, cell membrane.
UNIPROT #
别名
Blast 2 antibody
BLAST-2 antibody
Blast2 antibody
C type lectin domain family 4 member J antibody
C-type lectin domain family 4 member J antibody
C-type lectin domain family 4, member J antibody
CD 23 antibody
CD 23A antibody
CD23 antibody
CD23 antigen antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD23 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500386, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of CD23 was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500386, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
CD23 Recombinant Rabbit Monoclonal Antibody [PD00-03]
Application: IHC-P,mIHC
Reactivity: Human
Conjugate: unconjugated
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