CD11b Mouse Monoclonal Antibody [4-6]
Catalog# EM1701-42
CD11b Mouse Monoclonal Antibody [4-6]
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IHC-P
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IF-Cell
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FC
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WB
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Human
概述
产品名称
CD11b Mouse Monoclonal Antibody [4-6]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human CD11b aa 544-743 / 1,152.
种属反应性
Human
验证应用
IHC-P, IF-Cell, FC, WB
分子量
Predicted band size: 127 kDa
阳性对照
TF-1 cell lysate, THP-1 cell lysate, U-937 cell lysate, A431, LOVO, human tonsil tissue, human spleen tissue.
偶联
unconjugated
克隆号
4-6
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG2b
纯化方式
Protein G affinity purified.
应用稀释度
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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WB
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1:1,000
发表文章中的应用
发表文章中的种属
| Mouse | See 1 publications below |
靶点
功能
Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development.
背景文献
1. Jerke U et al.Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation. J. Biol. Chem. 286:7070-7081 (2011).
2. Bai M. et al. CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation. Blood 0:0-0 (2017).
序列相似性
Belongs to the integrin alpha chain family.
组织特异性
Predominantly expressed in monocytes and granulocytes. Expressed in neutrophils (at protein level).
亚细胞定位
Cell membrane.
UNIPROT #
别名
antigen CD11b (p170) antibody
Antigen CD11b p170 antibody
CD11 antigen like family member B antibody
CD11 antigen-like family member B antibody
CD11b antibody
CD11b/CD18 antibody
CD49d antibody
Cell surface glycoprotein MAC-1 subunit alpha antibody
Complement component 3 receptor 3 subunit antibody
Complement Component Receptor 3 Alpha antibody
展开图片
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Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (EM1701-42) at 1/1,000 dilution.
Lane 1: TF-1 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: U-937 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 30 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining CD11b (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining CD11b (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD11b antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD11b antibody. Counter stained with hematoxylin.
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Flow cytometric analysis of THP-1 cells with CD11b antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Optimized CAR-T therapy based on spatiotemporal changes and chemotactic mechanisms of MDSCs induced by hypofractionated radiotherapy
Author:
PMID: 37073129
应用: FC
反应种属: Mouse
发表时间: 2023 Jul
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Citation
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