概述
产品名称
Bcl-2 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human BCL-2 20-100 aa.
种属反应性
Human, Mouse
验证应用
WB, IF-Cell, FC
分子量
22/26 kDa
阳性对照
HL-60, mouse colon tissue lysate, LOVO, MCF-7, SH-SY-5Y.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:200-1:500
-
IF-Cell
-
1:50-1:200
-
FC
-
1:50-1:100
发表文章中的应用
靶点
功能
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release.
背景文献
1. Bruey J M et al. Bcl-2 and Bcl-XL regulate proinflammatory caspase-1 activation by interaction with NALP1. Cell 129:45-56 (2007).
2. Wei Y et al. JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy. Mol Cell 30:678-688 (2008).
序列相似性
Belongs to the Bcl-2 family.
组织特异性
Expressed in a variety of tissues.
翻译后修饰
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
亚细胞定位
Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.
UNIPROT #
别名
Apoptosis regulator Bcl 2 antibody
Apoptosis regulator Bcl-2 antibody
Apoptosis regulator Bcl2 antibody
AW986256 antibody
B cell CLL/lymphoma 2 antibody
B cell leukemia/lymphoma 2 antibody
Bcl-2 antibody
Bcl2 antibody
BCL2_HUMAN antibody
C430015F12Rik antibody
展开Apoptosis regulator Bcl 2 antibody
Apoptosis regulator Bcl-2 antibody
Apoptosis regulator Bcl2 antibody
AW986256 antibody
B cell CLL/lymphoma 2 antibody
B cell leukemia/lymphoma 2 antibody
Bcl-2 antibody
Bcl2 antibody
BCL2_HUMAN antibody
C430015F12Rik antibody
D630044D05Rik antibody
D830018M01Rik antibody
Leukemia/lymphoma, B-cell, 2 antibody
Oncogene B-cell leukemia 2 antibody
PPP1R50 antibody
Protein phosphatase 1, regulatory subunit 50 antibody
Bcl 2 antibody
折叠图片
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Western blot analysis of Bcl2 on different lysates using anti-Bcl2 antibody at 1/200 dilution.
Positive control:
Lane 1: HL-60
Lane 2: Mouse colon -
ICC staining Bcl2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining Bcl2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining Bcl2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Flow cytometric analysis of MCF-7 cells with Bcl2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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The Mitochondrial-Derived Peptide MOTS-c Alleviates Radiation Pneumonitis via an Nrf2-Dependent Mechanism
Author: Zhang Yanli,et al
PMID: NO PMID20240523
应用: WB
反应种属: Mouse
发表时间: 2024 May
-
Citation
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DNA hypomethylation of Syk induces oxidative stressand apoptosis via the PKCβ/P66shc signaling pathway indiabetic kidney disease
Author: Rui Zhang,et al
PMID: 38522019
应用: WB
反应种属: Human
发表时间: 2024 Mar
-
Citation
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Human Amniotic Epithelial Stem Cells Alleviate Autoimmune Premature Ovarian Insufficiency in Mice by Targeting Granulosa Cells via AKT/ERK Pathways
Author: Ye Xiaohang,et al
PMID: 38831179
应用: WB
反应种属: Mouse
发表时间: 2024 Jun
-
Citation
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BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiency
Author: Wang, F., Liu, Y., Ni, F., Jin, J., Wu, Y., Huang, Y., Ye, X., Shen, X., Ying, Y., Chen, J., Chen, R., Zhang, Y., Sun, X., Wang, S., Xu, X., Chen, C., Guo, J., & Zhang, D.
PMID: 36198708
应用: WB
反应种属: Mouse
发表时间: 2022 Oct
-
Citation
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Maternal consumption of a fermented diet protects offspring against intestinal inflammation by regulating the gut microbiota
Author: Wang, C., Wei, S., Liu, B., Wang, F., Lu, Z., Jin, M., & Wang, Y.
PMID: 35506256
应用: WB
反应种属: Pig
发表时间: 2022 May
-
Citation
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Prescription of Sageretia hamosa Brongn Relieved Goiter through Promoted Apoptosis of Thyroid Cells via miR-511-3p and PTEN/PI3K/Akt Pathway
Author: Deng, Y. L., Chen, S., Wang, H. T., Wang, B., & Xiao, K.
PMID: 34630982
应用: WB
反应种属: Rat
发表时间: 2021 Sep
-
Citation
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<html>Functional Analysis of the Promoter Regions of Two Apoptosis-Related Genes (<i>Bcl-2</i> and <i>Cycs</i>) and Their Regulation by Zn in Yellow Catfish. International Journal of Molecular Sciences, 22(12), 6291.</html>
Author: He, Y., Zhao, T., Chen, F., Song, C., Zhong, C., & Luo, Z.
PMID: 34208159
应用: WB
反应种属: Yellow catfish
发表时间: 2021 Jun
-
Citation