OXGR1 Recombinant Rabbit Monoclonal Antibody [JE64-04]
Catalog# HA721074
OXGR1 Recombinant Rabbit Monoclonal Antibody [JE64-04]
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WB
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Human
-
Mouse
-
Rat
概述
产品名称
OXGR1 Recombinant Rabbit Monoclonal Antibody [JE64-04]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human OXGR1 aa 171-220/337.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 38 kDa
阳性对照
Hela cell lysate, rat brain tissue lysate, mouse testis tissue lysates, rat testis tissue lysates.
偶联
unconjugated
克隆号
JE64-04
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500-1:2,000
靶点
功能
2-Oxoglutarate receptor 1 (OXGR1), also known as cysteinyl leukotriene receptor E (CysLTE) and GPR99, is a protein that in humans is encoded by the OXGR1 (also termed GPR99) gene. The Gene has recently been nominated as a receptor not only for 2-oxogluterate (see alpha-Ketoglutaric acid) but also for the three cysteinyl leukotrienes (CysLTs), particularly leukotriene E4 (LTE4) and to far lesser extents LTC4 and LTE4. Recent studies implicate GPR99 as a cellular receptor which is activated by LTE4 thereby causing these cells to contribute to mediating various allergic and hypersensitivity responses. Montelucast is in use to treat various conditions including asthma, exercise-induced bronchoconstriction, allergic rhinitis, primary dysmenorrhoea (i.e. dysmenorrhoea not associated with known causes; see dysmenorrhea#Causes), and urticaria. It has been presumed that this drug's beneficial effects in these diseases is due to its well-known ability to act as a receptor antagonist for the cysteinyl leukotriene receptor 1, i.e. it binds to but does not activate this receptor thereby interfering with LTD4, LTC4, and LTE4 provocative actions by blocking their binding to CysLTR1.
背景文献
1. Yuan Y. et. al. Exercise-induced alpha-ketoglutaric acid stimulates muscle hypertrophy and fat loss through OXGR1-dependent adrenal activation. EMBO J. 2020 Apr
2. Wang Q. et. al. Yak OXGR1 promotes fibroblast proliferation via the PI3K/AKT pathways. J Cell Biochem. 2019 Apr
亚细胞定位
Cell membrane.
别名
2 oxoglutarate receptor 1 antibody
2-oxoglutarate receptor 1 antibody
aKGR antibody
Alpha ketoglutarate receptor 1 antibody
Alpha-ketoglutarate receptor 1 antibody
G Protein Coupled Receptor 80 antibody
G Protein Coupled Receptor 99 antibody
G-protein coupled receptor 80 antibody
G-protein coupled receptor 99 antibody
GPR80 antibody
展开图片
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Western blot analysis of OXGR1 on different lysates with Rabbit anti-OXGR1 antibody (HA721074) at 1/500 dilution.
Lane 1: Hela cell lysate (10 µg/Lane)
Lane 2: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721074) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of OXGR1 on mouse testis tissue lysates with Rabbit anti-OXGR1 antibody (HA721074) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721074) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of OXGR1 on rat testis tissue lysates with Rabbit anti-OXGR1 antibody (HA721074) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721074) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"