MYL4 Rabbit Polyclonal Antibody
Catalog# HA500471
MYL4 Rabbit Polyclonal Antibody
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WB
-
IHC-P
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Human
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Mouse
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Rat
概述
产品名称
MYL4 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within human MYL4 aa 10-197 / 197.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
22 kDa
阳性对照
Mouse lung tissue lysate, human heart tissue lysate, rat lung tissue, rat heart tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IHC-P
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1:200-1:1,000
靶点
功能
Atrial Light Chain-1 (ALC-1), also known as Essential Light Chain, Atrial is a protein that in humans is encoded by the MYL4 gene. ALC-1 is expressed in fetal cardiac ventricular and fetal skeletal muscle, as well as fetal and adult cardiac atrial tissue. ALC-1 expression is reactivated in human ventricular myocardium in various cardiac muscle diseases, including hypertrophic cardiomyopathy, dilated cardiomyopathy, ischemic cardiomyopathy and congenital heart diseases. ALC-1 is expressed very early in skeletal muscle and cardiac muscle development; two E-boxes and CArG box in the MYL4 promoter region regulate transcription. ALC-1 expression in cardiac ventricles decreases in early postnatal development, but is highly expressed in atria throughout all of adulthood. Normal atrial function is essential for embryogenesis, as inactivation of the MYL7 gene was embryonic lethal at ED10.5-11.5. Evidence of ALC-1 isoform expression on contractile mechanics of sarcomeres came from experiments studying fibers from patients expressing a higher level of ALC-1 relative to VLC-1 in cardiac left ventricular tissue.
背景文献
1. Ghazizadeh Z. et. al. Metastable Atrial State Underlies the Primary Genetic Substrate for MYL4 Mutation-Associated Atrial Fibrillation. Circulation. 2020 Jan
2. Wang TY. et. al. Human cardiac myosin light chain 4 (MYL4) mosaic expression patterns vary by sex. Sci Rep. 2019 Sep
亚细胞定位
Myosin complex, cytosol, A band.
别名
ALC 1 antibody
ALC1 antibody
AMLC antibody
Atrial/embryonic alkali myosin light chain antibody
embryonic muscle/atrial isoform antibody
GT 1 antibody
GT1 antibody
MLC 1 antibody
MLC1 antibody
MYL 4 antibody
展开图片
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Western blot analysis of MYL4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500471, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse lung tissue lysate
Lane 2: Human heart tissue lysate -
Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-MYL4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500471, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-MYL4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500471, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"